SPIN-COLUMN-BASED PROTEIN EXTRACTION / CELL FRACTIONATION

Founded in 2010, Invent Biotechnologies is recognized worldwide for having revolutionized protein extraction and cell fractionation. As the inventor of a range of spin column-based disruptive technologies for protein research, we offer a simpler, smarter approach to sample preparation. This translates to streamlined workflows and more consistent results, providing superior data quality. By choosing our Minute™ Kits for protein extraction and cell fractionation, researchers can be confident that their samples accurately represent the endogenous profile of the cells or tissue under investigation. Requiring only a benchtop centrifuge, Minute™ Kits minimize experimental variables and deliver uncompromised material for more reliable data. The success of the Minute™ approach is evidenced by a growing number of publications, including citations in Nature, Science and Cell.

   

Minute™ Plant ER Enrichment Kit (20 preps)    (Invent Biotechnologies, Inc.) An understanding of endomembrane processes and their biological implication is important for understanding plant growth and development. Endosomes are a heterogeneous collection of organelles in the sorting and delivery of internalized material from cell surface and the transport of materials from Golgi to lysosome or vacuole.

Minute™ Plant Endosome Enrichment Kit (20 preps)    (Invent Biotechnologies, Inc.) An understanding of endomembrane processes and their biological implication is important for understanding plant growth and development. Endosomes are a heterogeneous collection of organelles in the sorting and delivery of internalized material from cell surface and the transport of materials from Golgi to lysosome or vacuole.

Minute™ Plant Cytosolic and Nuclear Protein Isolation Kit (Need 150 mg Raw Material, 20 preps)    (Invent Biotechnologies, Inc.) The kit is designed for rapid fractionation of fresh/frozen soft plant tissues, especially leaves. Unlike other commercial kit and Lab protocols, of which large amount of starting material (a few grams) and lengthy processing time are required, this kit only uses 100-200 mg starting material.

Minute™ Nuclear Proteasome Enrichment Kit (20 preps)    (Invent Biotechnologies, Inc.) The proteasome is a protein degradation system that requires metabolic energy and polymerization of ubiquitin that is different from lysosome-based protein degradation. The 26S proteasome is made up of two subcomplexes (a 20S proteasome and one or two19S regulatory particles).

Minute™ Chloroplast Isolation Kit (50 Preps)    (Invent Biotechnologies, Inc.) Invent Biotechnologies MinuteTM chloroplast isolation kit is composed of optimized chloroplast isolation buffers and filter cartridges with 2.0 ml collection tubes.

Minute™ Adipose Tissue Fractionation Kit (20 Preps)    (Invent Biotechnologies, Inc.) On average, total cellular proteins account for less than 2% of adipose tissue. Adipose tissue, especially white adipose tissue, has been recognized as an important endocrine and inflammation organ in addition to its energy storage function.

Minute™ Single Nuclei and Cytosol Isolation Kit for Adipose Tissues/Cultured Adipocytes (20 preps)    (Invent Biotechnologies, Inc.) Adipocytes are the major energy storage sites in the body and they also have critical endocrine functions. Therefore, understanding the development and function of adipocytes is essential to understanding metabolic homeostasis under physiological and pathological conditions.

Minute™ Single Nucleus Isolation Kit for Tissues/Cells    (Invent Biotechnologies, Inc.) Isolation and purification of nuclei from tissues and cultured cells are common lab practice. Isolated nuclei, especially single nucleus, are widely used for a variety of experiments such as FACS analysis, single nucleus analysis (such as RNA-seq and ATAC-seq)..

Minute™ Single Nucleus Isolation Kit for Neuronal Tissues/Cells (20 preps)    (Invent Biotechnologies, Inc.) Isolated nuclei are widely used for a variety of experiments such as FACS , single nucleus analysis (such as RNA-seq and ATAC-seq), immunofluorescence staining, cell cycle and apoptosis research. Single cell RNA-seq is a powerful technology for studying complex cellular composition of tissues.

Minute™ Detergent-Free Single Nuclei Isolation Kit (20 Preps)    (Invent Biotechnologies, Inc.) The detergent-free single nuclei isolation kit is designed to rapidly isolate intact nuclei from animal cultured cells or tissues (fresh or frozen). Intact nuclei can be isolated from the samples using proprietary spin-column-based technologies in less than 20 min without using tissue homogenizer and any detergents.