Minute™ Plant ER Enrichment Kit (20 preps)

Catalog# PR-048


For orders and inquiries

Contact Us


Isolation and enrichment of plant endoplasmic reticulum (ER) is essential for elucidation of ER related cellular functions. Traditional method for ER isolation is tedious and time consuming. In animal cells, ER is closely associated microtubules and Golgi apparatus are clustered at microtubule organizing center. In plant cells, the ER is associated with actin microfilaments and no microtubule organizing center is found near the nuclei. Due to structural characteristics of plan ER, it is much more difficult to isolate/enrich plant ER than that of animal samples. Minute™ plant ER enrichment kit was specially designed for plants. One to three-fold enrichment of ER fraction can be obtained from plant leave samples in about 1 hour. The enriched ER is essentially free from Golgi apparatus contamination. The enriched ER fraction can be used in plant protein trafficking studies.

Image:A. Ponceau S Stained Blot; B. Western blot with Anti-ER marker antibody (BIP2, Agrisera, Vannas, Sweden. AS 09481). Lanes 1&2: B. napus; Lanes 3&4: N. tabacum; Lanes 5&6: A. thaliana. Lanes 1, 3 & 5: Total Protein; Lanes 2, 4 & 6: ER fractions.

Kit Includes

Items Quantity
Buffer A 20 ml
Buffer B 1.2 ml
Buffer C 10 ml
Filter Cartridges with 2 ml Collection Tibe 20 Units
Plastic Rods 2 Units



Post Reviews

Leave a Reply

Your email address will not be published. Required fields are marked *

We're here to help

Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

Contact Tech Support

High Quality Guaranteed Product

Our products such as Elisa, Antibodies, Proteins, Peptides and sequencing kits are covered by Biolinkk quality warranty and will work as described in datasheet, a free replacement or money back is guaranteed if does not perform according to datasheet.

Learn More