Protein Extraction and Purification

BioVision's Protein Extraction and Purification Product Line contains antibiotics, cell lysis agents for bacterial cells, cell fraction kits, detergents, phosphatase and protease inhibitor cocktails, tag antibodies and protein quantitation kits. The fraction kits allow for separation of cytosolic, organellar, mitochondrial, nuclear, plasma membrane and cytoskeletal fractions. In addition, kits are included for isolation of genomic and/or mitochondrial DNA. Our detergent line includes high quality nonionic detergents (NP-40, Triton X-100, Tween-20, Tween-80, OG, OTG), a zwittergent detergents (CHAPS, ASB-14, Sulfobetaine 3-16) and an ionic detergent (SDS). The phosphatase and protease inhibitor cocktails are offered as ready to use formulations and encompass a wide variety and combinatorial forms which include both phosphatase and protease inhibitors. Our tag antibody line includes GST-, His-, HA-, S-, CBP-, DYDDDDK- and Myc-tag antibodies, while the anti-HA-tag antibodies are also offered with biotin or FITC conjugates. Finally, our protein quantification kits include both BCA-based and Bradford-type protein analysis.

Protein Extraction and Purification Products:

Urokinase Sepharose Beads (BioVision Inc.)

Beads for efficient and convenient cleavage of plasminogen and other recombinant fusion proteins containing a urokinase-specific cleavage site.

ToxOut™ Protein G (Sepharose) Spin Antibody Purification Kit (BioVision Inc.)

Simple, ready to use kit for endotoxin-free IgG purification and immunoprecipitation

ToxOut™ Protein G (Sepharose) Column (BioVision Inc.)

Ready to use column for purifying IgG from serum, ascites and cell culture media from various species such as human, mouse, rat, goat and rabbit

ToxOut™ Protein G (Sepharose) Antibody Purification Kit (BioVision Inc.)

Ready-to-use kit for endotoxin-free IgG purification and immunoprecipitation.

ToxOut™ Protein A (Sepharose) Spin Antibody Purification Kit (BioVision Inc.)

Simple, ready to use kit for endotoxin-free IgG purification and immunoprecipitation

ToxOut™ Protein A (Sepharose) Column (BioVision Inc.)

Ready to use column for purifying IgG from serum, ascites and cell culture media from various species such as human, mouse, rat, goat and rabbit.

ToxOut™ Protein A (Sepharose) Antibody Purification Kit (BioVision Inc.)

Ready-to-use kit for endotoxin-free IgG purification and immunoprecipitation.

Thrombin Sepharose Beads (BioVision Inc.)

Beads for convenient cleavage and purification of recombinant fusion proteins containing thrombin-specific cleavage sites.

Thrombin Cleavage Kit (BioVision Inc.)

Robust and efficient cleavage of tags from target proteins containing the Thrombin recognition sequence.

SUMO-GFP (Multi-Protease Control Protein) (BioVision Inc.)

A recombinant fusion protein that can be used as a cleavage control for testing the activity of SUMO Protease 1, Thrombin, Enterokinase, HRV14 3C (Precission Protease), Factor Xa and Tobacco Etch Virus Proteases (TEV).