Protein Extraction and Purification

BioVision's Protein Extraction and Purification Product Line contains antibiotics, cell lysis agents for bacterial cells, cell fraction kits, detergents, phosphatase and protease inhibitor cocktails, tag antibodies and protein quantitation kits. The fraction kits allow for separation of cytosolic, organellar, mitochondrial, nuclear, plasma membrane and cytoskeletal fractions. In addition, kits are included for isolation of genomic and/or mitochondrial DNA. Our detergent line includes high quality nonionic detergents (NP-40, Triton X-100, Tween-20, Tween-80, OG, OTG), a zwittergent detergents (CHAPS, ASB-14, Sulfobetaine 3-16) and an ionic detergent (SDS). The phosphatase and protease inhibitor cocktails are offered as ready to use formulations and encompass a wide variety and combinatorial forms which include both phosphatase and protease inhibitors. Our tag antibody line includes GST-, His-, HA-, S-, CBP-, DYDDDDK- and Myc-tag antibodies, while the anti-HA-tag antibodies are also offered with biotin or FITC conjugates. Finally, our protein quantification kits include both BCA-based and Bradford-type protein analysis.

Protein Extraction and Purification Products:

Hi-Bind™ Cibacron Blue-Agarose Beads (BioVision Inc.)

High binding beads for removal of albumin and numerous other proteins from multiple sample types.

Hemocyanin-Keyhole Limpet (KLH), Native (BioVision Inc.)

Hemocyanins are proteins that use copper binding sites to bind and transport oxygen in a variety of arthropods and mollusks. Hemocyanin is isolated from the hemolymph of the animals. Hemocyanin is one of the strongest antigens known. Hemocyanin has been in use as an immunological reagent for many years.

Hemocyanin-Keyhole Limpet (KLH) subunits, solution (BioVision Inc.)

Hemocyanins are proteins that use copper binding sites to bind and transport oxygen in a variety of arthropods and mollusks. Hemocyanin is isolated from the hemolymph of the animals. Hemocyanin is one of the strongest antigens known. Hemocyanin has been in use as an immunological reagent for many years.

Hemocyanin-Keyhole Limpet (KLH) subunits, powder (BioVision Inc.)

Hemocyanins are proteins that use copper binding sites to bind and transport oxygen in a variety of arthropods and mollusks. Hemocyanin is isolated from the hemolymph of the animals. Hemocyanin is one of the strongest antigens known. Hemocyanin has been in use as an immunological reagent for many years.

EZCut™ SUMO Protease 1 Agarose Beads (BioVision Inc.)

EZCut™ SUMO Protease 1 Agarose is prepared by covalent coupling of SUMO Protease 1 to activated 4% cross-linked agarose beads.

Protein Carbonyl Content Assay Kit (Fluorometric) (BioVision Inc.)

Protein carbonylation occurs when chronically elevated blood sugar levels create a microenvironment for sugars to covalently react with amino acids forming non-enzymatic adducts. Oxidative stress, the excessive production of ROS, can also give rise to the production of protein carbonyls.

Protein Carbonyl Content Assay Kit (BioVision Inc.)

Protein carbonyl groups are an important and immediate biomarker of oxidative stress. DNPH tagging of protein carbonyls has been one of the most common measures of oxidative stress. DNP hydrazones formed from the reaction are easily quantifiable at 375 nm.

His-tag Protein ELISA kit (BioVision Inc.)

A competitve ELISA kit for the quantitative measurement of Bacterial, insect and mammalian cell lysates, downstream analyses of purification of His-tag proteins, His-tag proteins used in protein-protein interaction studies
WARNING: This product can expose you to chemicals including TMB, which is [are] known to the State of California to cause cancer. For more information go to www.P65Warnings.ca.gov.

GST Tag ELISA Kit (BioVision Inc.)

Ready-to-use ELISA Kit to detect as low as 45 ng/ml of GST Tag within 3 hrs.
WARNING: This product can expose you to chemicals including TMB, which is [are] known to the State of California to cause cancer. For more information go to www.P65Warnings.ca.gov.

Extra Sense™ BCA Protein Assay Kit (BioVision Inc.)

BioVision’s Extra Sense™ BCA Protein Assay kit provides an ultra-sensitive method to detect and quantify total protein concentration even in the presence of detergents. The Kit has been optimized for determining total protein content in low protein concentration samples (0.5-40 μg/ml).