Protein phosphorylation is the most common and important form of reversible protein posttranslational modification (PTM), with up to 30% of all proteins being phosphorylated at any given time. Protein kinases (PKs) are the effectors of phosphorylation and catalyze the transfer of a γ-phosphate group from ATP to specific amino acids on proteins. Proteins are phosphorylated predominantly on Ser, Thr and Tyr residues, which account for 86, 12 and 2% respectively of the phosphoproteome, at least in mammals. In contrast, protein phosphatases (PPs) are the primary effectors of dephosphorylation, and are responsible for restoring the protein to its original dephosphorylated state. Phosphatases can be classified as non-specific phosphatases (e.g. acid or alkaline phosphatases), serine/threonine-specific, tyrosine-specific and dual-specific phosphatases (Ser/Thr & Tyr). A wide range of biological processes are controlled by phosphorylation and dephosphorylation of proteins. In order to understand the accurate phosphorylated status of a protein, it is important to preserve the phosphorylation state of the proteins during the extraction of phosphoproteins from tissue and cell sample lysates. BioVision introduces four different EZBlock™ Phosphatase Inhibitor Cocktails and two new Universal Protease and Phosphatase inhibitor cocktails, which would provide protection against both proteases and phosphatases during protein extraction and purification.

Phosphatase Inhibitor Cocktails Products:

EZBlock™ Universal Protease and Phosphatase Inhibitor Cocktail , EDTA-Free   (BioVision Inc.) A broad spectrum Protease and Phosphatase Inhibitor cocktail containing four protease and four phosphatase inhibitors. Also provided separately is a 0.5 M solution of EDTA Disodium.

EZBlock™ Universal Protease and Phosphatase Inhibitor Cocktail   (BioVision Inc.) A broad spectrum Protease and Phosphatase Inhibitor cocktail containing four protease and four phosphatase inhibitors. Also provided separately is a 0.5 M solution of EDTA Disodium.

EZBlock™ Phosphatase Inhibitor Cocktail XI   (BioVision Inc.) A cocktail consisting of six phosphatase inhibitors useful for the inhibition of acid and alkaline phosphatases, Ser/Thr phosphatases as well as protein tyrosine phosphatases (PTPs)

EZBlock™ Phosphatase Inhibitor Cocktail IV   (BioVision Inc.) A cocktail consisting of six phosphatase inhibitors useful for the inhibition of acid and alkaline phosphatases, Ser/Thr phosphatases as well as protein tyrosine phosphatases (PTPs)

EZBlock™ Phosphatase Inhibitor Cocktail III   (BioVision Inc.) A cocktail consisting of three phosphatase inhibitors useful for the inhibition of Ser/Thr and alkaline phosphatases. Recommended dilution: 1:50

EZBlock™ Phosphatase Inhibitor Cocktail II   (BioVision Inc.) A cocktail consisting of five phosphatase inhibitors useful for the inhibition of acid and alkaline phosphatases as well as protein tyrosine phosphatases (PTPs). Suitable for use with cell lysates and tissue extracts. Recommended dilution: 1:100.

EZBlock™ Phosphatase Inhibitor Cocktail I (50X)   (BioVision Inc.) A cocktail consisting of four phosphatase inhibitors useful for the inhibition of both serine/threonine and protein tyrosine phosphatases (PTPs). Suitable for use with cell lysates and tissue extracts.