ELISA

Enzyme-Linked Immunosorbent Assay (ELISA) is the assay format that is very popular in terms of efficiency, reproducibility, performance and validation in the plant pathogen diagnostic.

Bioreba ELISA reagents are delivered with Lot specific data sheets. Bioreba are committed to lot-to-lot consistency for increased test security.

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IgG and Conjugate are available for 100, 500 and for 1000 assays.

Reagent                Assays   
 10050010005000*
IgG0.025 ml0.1 ml0.2 ml1 ml
Conjugate0.025 ml0.1 ml0.2 ml1 ml

*For the six main potato viruses (PLRV, PVA, PVM, PVS, PVX and PVY), reagents are also available for 5000 assays.

Control                    Assays
 12
Positive control2.5 ml
Negative control2.5 ml


Component Assays
480 960 5000*
IgG 0.1 ml 0.2 ml 1 ml
Conjugate 0.1 ml 0.2 ml 1 ml
Positive control 2.5 ml 2.5 ml 5 x 2.5 ml
Negative control 2.5 ml 2.5 ml 5 x 2.5 ml
Microtiter plates 5 10 60
*For the six main potato viruses (PLRV, PVA, PVM, PVS, PVX and PVY), reagents are also available for 5000 assays.


Component Assays
96 480 960 5000*
IgG 25 ul 0.1 ml 0.2 ml 1 ml
Conjugate 25 ul 0.1 ml 0.2 ml 1 ml
Positive control 2.5 ml 2.5 ml 2.5 ml 1 ml
Negative control 2.5 ml 2.5 ml 2.5 ml 5 x 2.5 ml
Extraction buffer (10x)
 50 ml 250 ml 500 ml 5 x 500 ml
Coating buffer 1 tablet 1 tablet 2 tablets 10 tablets
Conjugate buffer (10x)
 2 ml 10 ml 20 ml 100 ml
Substrate buffer (5x)
 4 ml 20 ml 2 x 20 ml 200 ml
Washing buffer 1 tablet 1 pouch 2 pouches 2 containers
Substrate (pNPP)
4 tablets
(5 mg)
5 tablets
(20 mg)
10 tablets
(20 mg)
bulk powder
(5g)
Egg Albumin 5 g 25 g 50 g 250 g
Microtiter plates 1 5 10 60
*For the six main potato viruses (PLRV, PVA, PVM, PVS, PVX and PVY), reagents are also available for 5000 assays.
Crop Download pdf
Potatoes Pathogens_of_potatoes.pdf
Grapevines Pathogens_of_grapevines.pdf
Vegetables Pathogens_of_vegetables.pdf
Fruit Trees & Small Fruits Pathogens_of_fruittrees.pdf
Field Crops Pathogens_of_fieldcrops.pdf
Ornamentals Pathogens_of_ornamentals.pdf
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