SPIN-COLUMN-BASED PROTEIN EXTRACTION / CELL FRACTIONATION

Founded in 2010, Invent Biotechnologies is recognized worldwide for having revolutionized protein extraction and cell fractionation. As the inventor of a range of spin column-based disruptive technologies for protein research, we offer a simpler, smarter approach to sample preparation. This translates to streamlined workflows and more consistent results, providing superior data quality. By choosing our Minute™ Kits for protein extraction and cell fractionation, researchers can be confident that their samples accurately represent the endogenous profile of the cells or tissue under investigation. Requiring only a benchtop centrifuge, Minute™ Kits minimize experimental variables and deliver uncompromised material for more reliable data. The success of the Minute™ approach is evidenced by a growing number of publications, including citations in Nature, Science and Cell.

   

Minute™ Cell Suspension Isolation Kit from Fresh/Frozen Tissues (50 Preps)    (Invent Biotechnologies, Inc.) Obtaining quality cell suspension from fresh and/or frozen tissues is an important first step for subsequent analysis such as flow cytometry analysis (FACS) and nucleic acid purification. Cell suspensions can be isolated from tissues by one or a combination of the following three mechanisms: chemical tissue dissociation, enzymatic digestion and physical separation. Many methods are tedious and time consuming.

Minute™ Single Cell Isolation Kit for Fresh and Fixed Tissues (50 Preps)    (Invent Biotechnologies, Inc.) Minute™ Single Cell Isolation Kit is composed of optimized tissue disaggregation buffers and specially designed filter cartridges with 2.0 ml collection tubes.

Minute™ Cytosolic Proteasome Enrichment Kit (20 preps)    (Invent Biotechnologies, Inc.) The proteasome is a non-lysosome protein degradation system that requires metabolic energy and polymerization of ubiquitin. The 26S proteasome is made up of two subcomplexes (a 20S proteasome and one or two19S regulatory particles).

Minute™ Mitochondria Isolation Kit for Muscle Tissues/Cultured Muscle Cells    (Invent Biotechnologies, Inc.) Muscle cells have large number of mitochondria because they are continually used to move the body. Another mitochondrion-rich organ is the heart where mitochondria make up about 40% of the heart cells.

Minute™ Total Lipid Raft Isolation Kit for Mammalian Cells/Tissues    (Invent Biotechnologies, Inc.) Lipid rafts are small membrane domains containing high level of cholesterol and sphingolipids. Lipid rafts are found in plasma membrane (PM) and internal organellar membranes such as mitochondria-associated membrane (MAMs) and endoplasmic reticulum. They have been implicated in numerous cellular processes including but not limited to signal transduction...

Minute™ Plant Microsomal Membrane Extraction Kit (50 Preps)    (Invent Biotechnologies, Inc.) Isolation of microsomal membranes from plant tissues is a common procedure. The microsomal fraction of plant cell lysate is the focus of interest in many plant research projects and is believed to be enriched for plasma membranes, endoplasmic reticulum, Golgi apparatus, vacuolar membranes..

Minute™ Plant Plasma Membrane Protein Isolation Kit (50 Preps)    (Invent Biotechnologies, Inc.) Plasma membrane (PM) protein accounts for a small fraction of total cellular protein in plants but performs a very critical role in plant physiology. Isolation and purification of PM protein from plant tissues have been traditionally done by sucrose density ultracentrifugation and aqueous two-phase partitioning.

Minute™ High-Efficiency Saliva Exosome Isolation Kit (50 Preps)    (Invent Biotechnologies, Inc.) Diagnoses using exosomes derived from saliva have been attracting great attention in recent years due to the ease of sample collection. However, saliva samples are challenging when it comes down to exosome isolation. In addition to cells and cell debris..

Minute™ High-Efficiency Exosome Precipitation Reagent (20 ml)    (Invent Biotechnologies, Inc.) Exosomes are vesicles secreted by cells. They are present in a variety of body fluids such as serum, ascites, spinal cord fluid, urine, and saliva. Cultured cells also secrete significant number of exosomes. The size distribution of exosome is ranging from 30-120 nm.

Minute™ Yeast Mitochondria Enrichment Kit (50 Preps)    (Invent Biotechnologies, Inc.) Traditional protocol for yeast mitochondria isolation/extraction involves a range of centrifugation-based sub-cellular fractionation procedures. Typically, the techniques include spheroplast preparation, glass-bead lysis using a homogenization instrument, ..