SPIN-COLUMN-BASED PROTEIN EXTRACTION / CELL FRACTIONATION

Founded in 2010, Invent Biotechnologies is recognized worldwide for having revolutionized protein extraction and cell fractionation. As the inventor of a range of spin column-based disruptive technologies for protein research, we offer a simpler, smarter approach to sample preparation. This translates to streamlined workflows and more consistent results, providing superior data quality. By choosing our Minute™ Kits for protein extraction and cell fractionation, researchers can be confident that their samples accurately represent the endogenous profile of the cells or tissue under investigation. Requiring only a benchtop centrifuge, Minute™ Kits minimize experimental variables and deliver uncompromised material for more reliable data. The success of the Minute™ approach is evidenced by a growing number of publications, including citations in Nature, Science and Cell.

   

Minute™ Total Protein Extraction Kit for Blood Vessels (50 Preps)    (Invent Biotechnologies, Inc.) Blood vessels (arteries and veins) are made of specialized connective tissues. There are three layers of tissues in blood vessels: tunica, tunica media and endothelium. Due to their unique structures, blood vessels are notoriously difficult to homogenize.

Minute™ Protein Extraction Kit for Fixed and Embedded Tissues (20 Preps)    (Invent Biotechnologies, Inc.) Formalin-fixed and paraffin-embedded (FFPE) tissues are widely available and serve as a potentially rich source for biomedical research. However, protein extraction from FFPE tissue is very challenging due to formalin-mediated molecular cross-linking.

Minute™ Histone/DNA Binding Protein Extraction Kit (50 Preps)    (Invent Biotechnologies, Inc.) Histones and other DNA-binding proteins play an important role in chromosome organization, gene regulation and post-translational modification. Core components of histones can be covalently modified through methylation, acetylation, phosphorylation and sumoylation.

Minute™ Protein/Nucleic Acid Extraction Kit from Gels (20 Preps)    (Invent Biotechnologies, Inc.) Passive elution and electroelution are the most common methods for extracting proteins/nucleic acid from polyacrylamide/agarose gels. Protein extraction by passive elution usually takes overnight incubation. Eluted proteins are significantly diluted and require further concentration. Protein extraction by electroelution is faster (30 minutes to 2 hours) than passive elution, but it requires a special elution device that works less effectively for larger proteins (>70 kDa).

Minute™ Antibody Enhancer (30 ml)    (Invent Biotechnologies, Inc.) Minute™ Antibody Enhancer is able to significantly increase the sensitivity of commonly used immunoassays such as Western blotting, ELISA and immunohistochemistry.

Minute™ Total Protein Extraction Kit for Adipose Tissues/Cultured Adipocytes (20 Preps)    (Invent Biotechnologies, Inc.) Adipose tissue, especially white adipose tissue (WAT), has been recognized as an important endocrine and inflammation organ in addition to its energy storage function. Isolation and analysis of proteins from adipose tissues are increasingly critical for understanding many physiological/pathological conditions.

Minute™ Detergent-Free Protein Extraction Kit for Microbes with Thick Cell Walls (50 Preps)    (Invent Biotechnologies, Inc.) Protein extraction from cell-wall-containing microbes is a common procedure. The methods for protein extraction are usually harsh, tedious and unreliable. YD-016 provides a detergent-free, rapid and gentle way for extracting proteins from microbes with thick and strong cell walls.

Minute™ Total Protein Extraction Kit for Microbes with Thick Cell Walls (50 Preps)    (Invent Biotechnologies, Inc.) Protein extraction from cell-wall-containing microbes is a common procedure. The methods for protein extraction are usually harsh, tedious and unreliable. YT-015 provides an instrument-free, fast and gentle way for extracting proteins from microbes with thick and strong cell walls.

Minute™ Detergent-Free Protein Extraction Kit (For Animal Cultured Cells and Tissues) (50 Preps)    (Invent Biotechnologies, Inc.) Minute™ Detergent-Free Total Protein Extraction Kit is composed of optimized cell lysis buffer and protein extraction filter cartridges with 2.0 ml collection tubes. The kit is designed to quickly extract detergent-free total proteins from cultured cells (insect/mammalian/other cultured cells) and animal tissues.

Minute™ Albumin Depletion Reagent for Plasma and Serum (20 ml)    (Invent Biotechnologies, Inc.) Human/animal serum and plasma are the best sources of samples for biomarker identification. However, the presence of albumin in large amounts interferes with many assays and functional proteomic analysis. Serum albumin accounts for 50-70% of the total serum protein that causes loss of resolution for 1D and 2D electrophoresis.