Passive elution and electroelution are the most common methods for extracting proteins/nucleic acid from polyacrylamide/agarose gels. Protein extraction by passive elution usually takes overnight incubation. Eluted proteins are significantly diluted and require further concentration. Protein extraction by electroelution is faster (30 minutes to 2 hours) than passive elution, but it requires a special elution device that works less effectively for larger proteins (>70 kDa). The electro buffer usually contains detergent and other chemicals at a concentration that may interfere with downstream applications. Here we feature a rapid and instrument-free protein/nucleic acid extraction kit. Proteins/nucleic acid can be extracted from gels in less than 10 minutes with a high yield. The elution buffer can be a detergent-containing buffer or pure water depending on the method of gel staining. The elution volume is between 10 and 200 µl. Multiple gel pieces can be processed in a single tube, and the final protein concentration is relatively high.
|Items||Quantity||Filter Cartridges||20 Units|
|Microfuge Tubes||20 Units|
|Extraction Powder||2 grams|
1. Chowdhury, M. K., Jahan, M. S., Akhtar, S., Islam, M. A., Islam, M. A., Sikdar, B., & Hasan, M. F. Characterization of fungal pathogens causing diseases in bitter gourd and establishment of their eco-friendly control measure.
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