Minute™ Protein Extraction Kit for Fixed and Embedded Tissues (20 Preps)
Manual & Protocol (PDF) | Material Safety Data Sheets (MSDS)(PDF)
View All Related Products
Description
Formalin-fixed and paraffin-embedded (FFPE) tissues are widely available and serve as a potentially rich source for biomedical research. However, protein extraction from FFPE tissue is very challenging due to formalin-mediated molecular cross-linking. Traditional methods for protein extraction from FFPE tissues involve repeated deparaffinization and rehydration using organic solvents followed by protein extraction at a high temperature with or without an optional sonication step. These methods can be effective but are tedious and time-consuming (3-4 hours). Minute™ Protein Extraction Kit for Fixed and Embedded Tissues provides a simple and rapid way to extract protein from FFPE tissue without organic solvent deparaffinization. The whole procedure can be completed in less than one hour with a protein yield of 2-3 mg/ml.
Kit Includes
Items | Quantity | Buffer A | 25 ml |
Buffer B | 25 ml |
1.5 ml Microfuge Tubes | 20 units |
Pestles | 2 units |
References
-
Post Reviews
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact Tech Support
High Quality Guaranteed Product
Our products such as Elisa, Antibodies, Proteins, Peptides and sequencing kits are covered by Biolinkk quality warranty and will work as described in datasheet, a free replacement or money back is guaranteed if does not perform according to datasheet.
Learn More