Protein extraction from cell-wall-containing microbes is a common procedure. The methods for protein extraction are usually harsh, tedious and unreliable. YD-016 provides a detergent-free, rapid and gentle way for extracting proteins from microbes with thick and strong cell walls. These microbes include yeast, filamentous fungus, gram-positive and -negative bacteria, insect eggs, microalgae and more. This kit contains optimized detergent-free and EDTA-free protein extraction buffers. The whole procedure takes less than 10 minutes and the protein yield is in the range of 2-4 mg/ml with unbiased protein representation. This kit contains a single-tube protocol and works well for both log-phase and stationary-phase microbes. The materials provided are sufficient for 50 extractions.
|Items||Quantity||Buffer A||20 ml|
|Buffer B||20 ml|
|Protein Extraction Powder||5 grams|
|1.5 ml Microfuge Tube||50 units|
1. Peng, G., Hou, X., Zhang, W., Song, M., Yin, M., Wang, J., ... & Li, X. (2018). Alkyl rhamnosides, a series of amphiphilic materials exerting broad-spectrum anti-biofilm activity against pathogenic bacteria via multiple mechanisms. Artificial cells, nanomedicine, and biotechnology, 1-16.
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