SPIN-COLUMN-BASED PROTEIN EXTRACTION / CELL FRACTIONATION

Founded in 2010, Invent Biotechnologies is recognized worldwide for having revolutionized protein extraction and cell fractionation. As the inventor of a range of spin column-based disruptive technologies for protein research, we offer a simpler, smarter approach to sample preparation. This translates to streamlined workflows and more consistent results, providing superior data quality. By choosing our Minute™ Kits for protein extraction and cell fractionation, researchers can be confident that their samples accurately represent the endogenous profile of the cells or tissue under investigation. Requiring only a benchtop centrifuge, Minute™ Kits minimize experimental variables and deliver uncompromised material for more reliable data. The success of the Minute™ approach is evidenced by a growing number of publications, including citations in Nature, Science and Cell.

   

Minute™ Denaturing Protein Solubilization Reagent (10 ml)    (Invent Biotechnologies, Inc.) This reagent is formulated for dissolving protein pellets using protein kits from Invent Biotechnologies (Cat# Nos. SM-005, SM-005-P, CP-011, NE-013, YM-017, MM-018 and AF-023) and other companies. Solubilized proteins are denatured due to the presence of an anionic detergent (SDS).

Protein Extraction Kit for Hair and Nails (20 Preps)    (Invent Biotechnologies, Inc.) Proteins account for about 80% of keratinized animal tissues such as hair, nail, horn and wool. These tissues contain mainly hard keratins made of several distinct types of protein with a molecular weight ranging from 10 to 135 kDa.

Minute™ Total Protein Extraction Kit for Tendons (50 Preps)    (Invent Biotechnologies, Inc.) A tendon/ligament is a fibrous connective tissue that attaches muscles to bone. Histologically tendons consist of dense regular collagen fibers that are very tough and notoriously difficult to homogenize. It is also very difficult to lyse the tendon tissues for total protein extraction. Traditional solution-based protein extraction methods, such as RIPA, are inefficient and the protein yield is very low.

Minute™ Total Protein Extraction Kit for Skin Tissue (50 Preps)    (Invent Biotechnologies, Inc.) Skin tissue is made up of epidermis, dermis and subcutaneous fat. Because of its unique structure, skin tissue is notoriously difficult to homogenize. It is also very difficult to lyse the cells in the tissue for total protein extraction. Traditional solution-based protein extraction methods, such as RIPA, are inefficient and the yield is very low. The profile of extracted protein is also incomplete with solution-based methods.

Minute™ Total Protein Extraction Kit for Plant Tissues (50 Preps)    (Invent Biotechnologies, Inc.) Minute™ Total Protein Extraction Kit for Plant Tissues is composed of optimized protein extraction buffer and protein extraction filter cartridges with 2.0 ml collection tubes. The kit is designed to rapidly extract denatured or native proteins from plant tissues (leaves, seeds, soft stems, roots, etc.).

Minute™ Total Protein Extraction Kit for Muscles (50 Preps)    (Invent Biotechnologies, Inc.) Skeletal and cardiac muscles are highly specialized tissues that are made of well-arranged muscle fibers. An individual muscle may be made up of hundreds or even thousands of muscle fibers bundled together and wrapped in a connective tissue covering.

Minute™ Total Protein Extraction Kit for Mass Spectrometry (50 Preps)    (Invent Biotechnologies, Inc.) Mass spectrometry has become a popular method for analysis and characterization of proteins. RIPA buffer-based protein extraction suffers the disadvantages of incomplete protein extraction and some components may not be compatible with certain downstream applications.

Minute™ Total Protein Extraction Kit for Intestines and Mesentery (50 Preps)    (Invent Biotechnologies, Inc.) The intestines are a long and continuous tube that run from stomach to anus. Mesentery is a fold of tissue that attaches intestines to the body wall. Structurally, intestines and mesentery are very different. However, from a protein extraction point of view, they have one thing in common: they are very tough and hard to homogenize.

Minute™ Total Protein Extraction Kit for Insects (50 Preps)    (Invent Biotechnologies, Inc.) Despite significant variation in body organization, insects all have the same general body structure. They have segmented bodies divided into three regions: head, thorax and abdomen. The body segments are protected by a hard exoskeleton or cuticles.

Minute™ Total Protein Extraction Kit for Bone Tissue (50 Preps)    (Invent Biotechnologies, Inc.) Bone tissues are commonly used for research. There are two types of bone tissues: compact and spongy. Cells in bone tissues are tightly packed and, because of their unique structures, it is very difficult to efficiently extract total protein from them.