Minute™ Detergent-Free Single Nuclei Isolation Kit (20 Preps)

Minute™ Detergent-Free Single Nuclei Isolation Kit (20 Preps)

Manual & Protocol (PDF)   |   Material Safety Data Sheets (MSDS)(PDF)

View All Related Products

Catalog# NI-024


For orders and inquiries

Contact Us


Watch the newest Video on JoVE

The detergent-free single nuclei isolation kit is designed to rapidly isolate intact nuclei from animal cultured cells or tissues (fresh or frozen). Intact nuclei can be isolated from the samples using proprietary spin-column-based technologies in less than 20 min without using tissue homogenizer and any detergents. Traditional method for nuclei isolation involves the use of non-ionic detergent, which has tendency to cause unwanted nuclear aggregation. It is unclear why some nuclei are aggregated while others are not. It is also unknown whether non-aggregated nuclei are unbiased representation of the whole nuclei population. Detergents also have the potential to damage the nuclear envelope resulting in leakage of nuclear matrix materials. For some cell/tissue types, nuclear envelop proteins could also be stripped off by the detergents. MinuteTM Detergent-Free Nuclei Isolation Kit provides a whole new way for nuclei isolation in contrast to traditional methods.

How it works: cells/tissues are first sensitized by buffer A before passing through the proprietary filter in a zigzag manner when high-speed centrifugal force is applied. The cells are ruptured when passing through the filter leaving intact native nuclei in the flow through. The nuclei are separated from other small cell debris by low speed centrifugation using the proprietary buffer B. The native and intact nuclei isolated can be used for a variety of downstream applications that include but not limited to: FACS analysis, single nucleus analysis (such as RNA-seq and ATAC-seq), immunofluorescence staining, cell cycle analysis and/or apoptosis research.

NOTE: We also offer the following single nucleus isolation kits with much cleaner background if the presence of detergent is not a concern:

SN-047: Minute™ Single Nucleus Isolation Kit for Tissues/Cells

BN-020: Minute™ Single Nucleus Isolation Kit for Neuronal Tissues/Cells

AN-029: Minute™ Nuclei and Cytosol Isolation Kit for Adipose Tissues/Cultured Adipocytes

Image:Nuclei Isolated from zebrafish brain tissue Using NI-024 and stained with DAPI (False-coloured in green) (Courtesy Sema Elif Eski, Singh Lab, Université Libre de Bruxelles).

Kit Includes

Items Quantity
Buffer A 15 ml
Buffer B 30 ml
Protein Extraction Filter Cartridges 20 Units
Pestles 2 Units
Collection Tubes 20 Units


1. Boerhan, R., Sun, W., Tian, N., Wang, Y., Lu, J., Li, C., ... & Zhou, Q. X. (2019). Fluorination on non-photolabile dppz ligand improving Ru (II) complex-based photoactivated chemotherapy. Dalton Transactions.

2. Eski, S.E., Dubois, C., Singh, S.P. NucleiIsolation from Whole Tissue usinga Detergent and Enzyme-FreeMethod. J. Vis. Exp. (160), e61471,doi:10.3791/61471 (2020).

Post Reviews

Leave a Reply

Your email address will not be published. Required fields are marked *

We're here to help

Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

Contact Tech Support

High Quality Guaranteed Product

Our products such as Elisa, Antibodies, Proteins, Peptides and sequencing kits are covered by Biolinkk quality warranty and will work as described in datasheet, a free replacement or money back is guaranteed if does not perform according to datasheet.

Learn More