Protein Purification

BioVision's Protein Extraction and Purification Product Line contains antibiotics, cell lysis agents for bacterial cells, cell fraction kits, detergents, phosphatase and protease inhibitor cocktails, tag antibodies and protein quantitation kits. The fraction kits allow for separation of cytosolic, organellar, mitochondrial, nuclear, plasma membrane and cytoskeletal fractions. In addition, kits are included for isolation of genomic and/or mitochondrial DNA. Our detergent line includes high quality nonionic detergents (NP-40, Triton X-100, Tween-20, Tween-80, OG, OTG), a zwittergent detergents (CHAPS, ASB-14, Sulfobetaine 3-16) and an ionic detergent (SDS). The phosphatase and protease inhibitor cocktails are offered as ready to use formulations and encompass a wide variety and combinatorial forms which include both phosphatase and protease inhibitors. Our tag antibody line includes GST-, His-, HA-, S-, CBP-, DYDDDDK- and Myc-tag antibodies, while the anti-HA-tag antibodies are also offered with biotin or FITC conjugates. Finally, our protein quantification kits include both BCA-based and Bradford-type protein analysis.

Protein Purification Products:

Hi-Bind™ Cibacron Blue-Agarose Beads (BioVision Inc.)

High binding beads for removal of albumin and numerous other proteins from multiple sample types.

Hemocyanin-Keyhole Limpet (KLH), Native (BioVision Inc.)

Hemocyanins are proteins that use copper binding sites to bind and transport oxygen in a variety of arthropods and mollusks. Hemocyanin is isolated from the hemolymph of the animals. Hemocyanin is one of the strongest antigens known. Hemocyanin has been in use as an immunological reagent for many years.

Hemocyanin-Keyhole Limpet (KLH) subunits, solution (BioVision Inc.)

Hemocyanins are proteins that use copper binding sites to bind and transport oxygen in a variety of arthropods and mollusks. Hemocyanin is isolated from the hemolymph of the animals. Hemocyanin is one of the strongest antigens known. Hemocyanin has been in use as an immunological reagent for many years.

Hemocyanin-Keyhole Limpet (KLH) subunits, powder (BioVision Inc.)

Hemocyanins are proteins that use copper binding sites to bind and transport oxygen in a variety of arthropods and mollusks. Hemocyanin is isolated from the hemolymph of the animals. Hemocyanin is one of the strongest antigens known. Hemocyanin has been in use as an immunological reagent for many years.

EZCut™ SUMO Protease 1 Agarose Beads (BioVision Inc.)

EZCut™ SUMO Protease 1 Agarose is prepared by covalent coupling of SUMO Protease 1 to activated 4% cross-linked agarose beads.

EZCut™ HRV14 3C (Precission) Protease Agarose Beads (BioVision Inc.)

EZCut™ HRV14 3C (Precission) Protease Agarose is prepared by covalent coupling active HRV14 3C Protease to activated 4% cross-linked agarose beads.

Core Streptavidin (BioVision Inc.)

A non-glycosylated protein which is widely used to bridge biotynlated probes and enzymes.

Ammonium sulfate saturated solution (BioVision Inc.)

Convenient, ready to use sterile solution for Purification/Fractionation of proteins

7” Polypropylene Disposable Gravity Column (BioVision Inc.)

Disposable polyproplyene columns for general chromatography

Maleimide Activated KLH-Peptide Conjugation Kit (BioVision Inc.)

Maleimide Activated KLH is a commonly used carrier protein for haptens, (small or low molecular weight molecules and peptides used to enable the immune response). Maleimide Activated KLH is the most popular carrier protein used for antibody production