Ammonium sulfate saturated solution
Datasheet (PDF) | Safety Data Sheets (MSDS)(PDF)
View All Related Products
Description
Protein solubility is dependent on the ionic strength of the solution, among other factors. Two distinct phenomenon of protein solubilization are seen in general: 1) Salting in: At low salt concentrations, the solubility of the protein increases with increasing salt concentration (i.e. increasing ionic strength). 2) Salting out: As the salt concentration (ionic strength) increases, the solubility of the protein begins to decrease. At certain ionic strength, the protein will be almost completely precipitate from the solution. Since proteins differ markedly in their solubility at high ionic strength, salting-out is a very useful procedure to assist in the purification of a given protein. Ammonium sulfate has been widely used in salting out for protein purification, as it is very water soluble, forms two ions high in the Hofmeister series1, and has no adverse effects upon enzyme activity. It is generally used as a saturated aqueous solution which is diluted to the required concentration and expressed as a percentage concentration of the saturated solution (a 100% solution). While ammonium sulfate fractionation is a low resolution technique, it is used successfully to reduce the complexity and decrease the sample volume on a daily basis by many scientists.
Datasheet
Cat # +Size | 7096-1L |
---|---|
Size | 1 liter |
Highlights | Convenient, ready to use Sterile Ammonium sulfate at 4.32 M. Can be used for: • Purification/Fractionation of proteins • Concentration of protein solutions• Buffer exchange • Remove interference of small molecules from protein/enzyme solutions. Compatible with: • Serum, plasma & other body fluids • Tissues extracts, animal, plant, bacterial extracts, etc. • Cell culture supernatants • Fermentation media |
Storage Conditions | RT |
Shipping Conditions | RT |
USAGE | For Research Use Only! Not For Use in Humans. |
FAQ
-
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact Tech Support
High Quality Guaranteed Product
Our products such as Elisa, Antibodies, Proteins, Peptides and sequencing kits are covered by Biolinkk quality warranty and will work as described in datasheet, a free replacement or money back is guaranteed if does not perform according to datasheet.
Learn More