Protein Purification

BioVision's Protein Extraction and Purification Product Line contains antibiotics, cell lysis agents for bacterial cells, cell fraction kits, detergents, phosphatase and protease inhibitor cocktails, tag antibodies and protein quantitation kits. The fraction kits allow for separation of cytosolic, organellar, mitochondrial, nuclear, plasma membrane and cytoskeletal fractions. In addition, kits are included for isolation of genomic and/or mitochondrial DNA. Our detergent line includes high quality nonionic detergents (NP-40, Triton X-100, Tween-20, Tween-80, OG, OTG), a zwittergent detergents (CHAPS, ASB-14, Sulfobetaine 3-16) and an ionic detergent (SDS). The phosphatase and protease inhibitor cocktails are offered as ready to use formulations and encompass a wide variety and combinatorial forms which include both phosphatase and protease inhibitors. Our tag antibody line includes GST-, His-, HA-, S-, CBP-, DYDDDDK- and Myc-tag antibodies, while the anti-HA-tag antibodies are also offered with biotin or FITC conjugates. Finally, our protein quantification kits include both BCA-based and Bradford-type protein analysis.

Protein Purification Products:

Streptavidin-Sepharose Beads (BioVision Inc.)

Sepharose beads conjugated to streptavidin for binding to free biotin or biotin based products.

Streptavidin coated 96-well Plate (BioVision Inc.)

BioVision’s High Sensitivity Streptavidin plates (clear, 12x8-Well Strips), are ready-to-use biotin-binding microplates. Proprietary coating of the plates with highly pure streptavidin allows the user to detect biotin with high sensitivity and low background. The microplates are pre-blocked with BioVision’s unique blocking buffer. The plates allow detection of as low as 1 ng/ml biotinylated rabbit IgG. The plates are compatible with fluorescent, colorimetric or chemiluminescent detection, using biotinylated ligands such as biotin-labeled oligonucleotides, peptides /proteins or IgG etc.

Ready-to-Use Ni-IDA Spin Purification Kit (BioVision Inc.)

Kit with Hi-Bind™ Ni QR Agarose Bead columns, & loading, washing & elution buffers for small scale purification of poly-histidine tagged proteins

Ready-to-Use Ni QR Agarose Beads Buffer Kit (BioVision Inc.)

Basic research of the physico-chemical properties as well as the activity of proteins is impossible without their isolation and purification. Majority of protein studies are carried out on tagged recombinant proteins expressed in various host organisms. Over 50% of these are expressed as fusions with poly-histidine purification tags.

Plasmin Sepharose Beads (BioVision Inc.)

Beads for efficient and convenient cleavage of recombinant fusion proteins containing plasmin-specific cleavage site.

Ni-IDA Spin Columns (BioVision Inc.)

Columns with Hi-Bind™ Ni QR Agarose Beads for small scale purification of poly-histidine tagged proteins

Multipurpose Mini Spin Columns (BioVision Inc.)

Columns aiding in IP, antibody purification, protein desalting etc.

Melibiose-Sepharose® Beads (BioVision Inc.)

Reusable high binding capacity agarose for selective binding and rapid purification of all types of immunoglobulins under universal and mild conditions.

Hi-Bind™ T-Gel-Agarose (BioVision Inc.)

Reusable high binding capacity agarose for selective binding and rapid purification of all types of immunoglobulins under universal and mild conditions.

Hi-Bind™ Ni QR Agarose Beads (BioVision Inc.)

Reusable Ni ion conjugated agarose beads with > 60 mg/ml binding capacity & > 4cm/min flow rate for purification of metal ions binding proteins