Streptavidin-Sepharose Beads
Datasheet (PDF) | Safety Data Sheets (MSDS)(PDF)
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Description
Streptavidin, a protein from Streptomyces avidinii, is known to bind to biotin with very high affinity (dissociation constant 10-14 M). It has a tetrameric structure in which each molecule of Streptavidin binds to four molecules of biotin. These interactions have been utilized to isolate variety of biotin labeled proteins, antibodies and other biomolecules (BioVision Cat #s 2322, 2323, 2326, 2345-2347). BioVision’s leak-resistant Streptavidin conjugated beads are prepared by covalent conjugation of Streptavidin to highly cross-linked Sepharose beads by a proprietary method.
Datasheet
| Cat # +Size | 6565-2 (2 ml) 6565-5 (5 ml) 6565-10 (10 ml) |
|---|---|
| Highlights | BINDING CAPACITY: The binding capacity of the Streptavidin-Sepharose beads is 20-40 μg for free biotin (a) or 2-3 mg for biotinylated BSA (b) per ml of resin. a: As determined by Biovision’s Biotin Assay Kit, Catalog # K811-50,100. b: BioVision’s Biotinylated BSA, Catalog # 7097. |
| Storage Conditions | 4°C |
| Shipping Conditions | Gel Pack |
| USAGE | For Research Use Only! Not For Use in Humans. |
FAQ
What is the size of the sepharose beads and how homogeneous the size distribution of the beads is?
The sepharose bead size is between 45 -165 µm and has a uniform distribution.
What is the source of Streptavidin?
It is a recombinant protein expressed in E.coli.
Can we reuse the beads?
We do not recommend re-using the beads. As the binding between the streptavidin and biotin is very strong and not reversible, it is not suggested to reuse the beads.
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Our products such as Elisa, Antibodies, Proteins, Peptides and sequencing kits are covered by Biolinkk quality warranty and will work as described in datasheet, a free replacement or money back is guaranteed if does not perform according to datasheet.
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