Nucleic Acid Purification

Quick-DNA Plant/Seed 96 Kit

The Quick-DNA Plant/Seed 96 Kit is a plant DNA isolation kit designed for the simple, rapid isolation of inhibitor-free, PCR-quality DNA from a variety of plant sample sources including leaves, stems, buds, flowers, fruit, seeds, etc. The procedure is easy and can be completed in minutes: plant samples are rapidly and efficiently lysed by bead beating with our state of the art..

Quick-DNA Plant/Seed Miniprep Kit

The Quick-DNA Plant/Seed Miniprep Kit is a plant DNA isolation kit designed for the simple, rapid isolation of inhibitor-free, PCR-quality DNA from a variety of plant sample sources including leaves, stems, buds, flowers, fruit, seeds, etc.

Quick-DNA Plant/Seed Kits

Q1: Can you provide a list of the tested plant species? We currently do not have a list of plants, aside from what we have shown in the protocols: – A.thaliana – Juniper – Milkweed Leaf – Milkweed Leaflet – Milkweed Pre-Flowering Bud – Corn Kernel – Sunflower Seed – Nicotiana sp. Q2: My lysate seems viscous. What is causing this to happen? How can I fix this? A viscous sample can indicate incomplete sample lysis. Try using less of your sample and optimize bead beating conditions (duration, speed, time) to ensure samples are thoroughly lysed. After bead beating, pellet the cell debris before moving on. Adding more Genomic Lysis buffer to the lysate can help dilute and deproteinate the sample, making the sample less viscous and more suitable for DNA recovery. Q3: Are there any tips in optimzing bead beating conditions? We have validated our kits with both high-speed homgenizers and low-speed disruptors. We highly recommend users to optimize their bead beating conditions for their own instruments. We recommend using a 2 ml-tube adapter to ensure that the bead beating is efficent (do not use adapters made of foam). For high-speed homogenizers, we recommend a total of 5 mins bead beating (1 min interval at 6.5 m/s with 5 mins rest, repeat 5 times). For low-speed cell disruptors, we recommend 30 mins at max speed. Q4: What is the purpose of Zymo-Spin III-HRC step? Environmental samples often contain inhibitors such as polyphenolics, humic/fulvic acids, tannins, melanins, etc. that often co-purify and affect downstream applications such as PCR. The Zymo-Spin III-HRC column removes these polyphenolic PCR inhibitors to recover DNA that is ready for sensitive downstream applications such as Next-Gen Sequencing, quantitative PCR, etc. Q5: Is it necessary to add beta-mercaptoethanol? Can this step be substituted or omitted? Addition of beta-mercaptoethanol is recommended to enhance sample lysis, but can be substituted with dithiothreitol (DTT, final concentration of 10 mM). However, if bead beating is optimized and lysis is efficient, the addition of BME is not necessary and can be omitted. Q6: When can an RNase A treatment be implemented in the protocol? The Quick-DNA kits recover RNA-free genomic DNA. The selective chemistry allows for binding of double stranded DNA to the column and for RNA to flow through. No RNase A treatment is required when processing samples within kit capacity.

ZymoBIOMICS DNA Miniprep Kit

The ZymoBIOMICS DNA Kits are microbial DNA purification kits designed for purifying DNA from a variety of sample inputs that is immediately ready for microbiome or metagenome analyses. The ZymoBIOMICS lysis system eliminates bias associated with unequal lysis efficiencies of different organisms (e.g. Gram-negative/positive bacteria, fungus, protozoans, and algae) making it ideal for microbial community profiling. Uniform mechanical lysis of all microbes is achieved by bead beating with the innovative ultra-high density BashingBeads. This kit is equipped with our OneStep PCR Inhibitor removal technology, enabling PCR amplification from DNA derived from inhibitor-rich environmental samples. Purified DNA is ideal for all downstream applications including PCR, arrays, 16S rRNA gene sequencing, and shotgun sequencing. DNA Size is 15-20 kb.

ZymoBIOMICS DNA/RNA Miniprep Kit

The ZymoBIOMICS DNA/RNA Miniprep Kit is designed for purifying DNA and RNA from a wide array of sample inputs (e.g. feces, soil, plant, water, and biofilms) that is ready for microbiome or metagenome analyses. The ZymoBIOMICS innovative lysis system eliminates bias associated with unequal lysis efficiencies of different organisms (e.g. Gram-negative/positive bacteria, fungus, protozoans, and algae). The provided DNA/RNA Shield preserves nucleic acids at ambient temperatures, providing an unbiased molecular snapshot of the sample. The procedure uses Zymo-Spin Column technology that results in high-quality DNA and total RNA (including small RNAs 17-200 nt) that is free of PCR inhibitors (e.g. polyphenols, humic acids, and fulvic acids). Ready for RT-PCR, arrays, sequencing, etc.

SARS-CoV-2 Quantification Standards

The SARS-CoV-2 Quantification Standards are a set of RNA standards that can be used to quantify the concentrations of RNA from the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) which is responsible for the coronavirus disease (COVID-19). These RNA standards contain a region of the viral nucleocapsid (N) gene and a host-specific target region (human RNase P gene) to assess standard integrity. The kit also includes a No-Template Control to confirm the absence of contamination in the reagents.

ZymoScript One-Step RT-qPCR Kit

ZymoScript One-Step RT-qPCR Kit is an optimized RT-qPCR kit that contains all the necessary components for fast reverse transcription and robust qPCR amplification in a single tube. The One-Step RT-qPCR kit is compatible with both SYBR Green and TaqMan probe-based assays for ultimate flexibility. The visual tracking dye included in the master mix allows accurate reaction set-up.

ZymoScript RT PreMix Kit

ZymoScript RT PreMix is an optimized reverse transcription kit containing all the necessary components for quick and efficient cDNA synthesis. This reverse transcription mix includes random hexamer and oligo dT primers, dNTPs, RNase inhibitors, reverse transcriptase, and an optimized reaction buffer which includes a visual tracking dye for accurate reaction set-up. This is the kit of choice for analysis of RNA via 2-step RT-qPCR.

DNA/RNA Shield SafeCollect Swab Collection Kit

The DNA/RNA Shield SafeCollect Swab Collection Kit is a user-friendly collection kit for stabilizing the nucleic acid content of samples collected with a swab. DNA/RNA Shield completely inactivates harmful pathogens in the sample, and stabilizes the RNA in a sample for up to 30 days at room temperature. The SafeCollect design eliminates the risk of spilling the stabilization reagent during use, and is ideal for applications that require at-home self collection.

DNA/RNA Shield SafeCollect Saliva Collection Kit

The DNA/RNA Shield SafeCollect Saliva Collection Kit is a user-friendly saliva collection kit for stabilizing the nucleic acid content of saliva and sputum samples. DNA/RNA Shield completely inactivates harmful pathogens in the sample, and stabilizes the RNA in a sample for up to 30 days at room temperature. The SafeCollect design eliminates the risk of spilling the stabilization reagent during use, and is ideal for applications that require at-home self collection.