Quick-DNA Plant/Seed Miniprep Kit
D6020
Cat # | Name | Size |
---|---|---|
D6020 | Quick-DNA Plant/Seed Miniprep Kit | 50 Preps |
highlights
- ✓ Rapid and simple method for DNA isolation from tough-to-lyse plant and seed samples.
- ✓ Ultra-high density BashingBeads are fracture resistant and chemically inert.
- ✓ Zymo-Spin column technology coupled with filtration removes polyphenolic PCR inhibitors from the DNA product.
The Quick-DNA Plant/Seed Miniprep Kit is a plant DNA isolation kit designed for the simple, rapid isolation of inhibitor-free, PCR-quality DNA from a variety of plant sample sources including leaves, stems, buds, flowers, fruit, seeds, etc. The procedure is easy and can be completed in minutes: plant samples are rapidly and efficiently lysed by bead beating with our state of the art, ultra-high density BashingBeads. Polysaccharides, lipids, and polyphenols/tannins are removed from the DNA using our Zymo-Spin column technology. The eluted DNA is ideal for downstream molecular-based applications including PCR, arrays, etc.
Applicable For | All sensitive downstream applications such as qPCR and Next-Generation Sequencing. |
---|---|
Elution Volume | ≥ 50 µl |
Equipment | Microcentrifuge, vortex, cell disruptor/pulverizer |
Processing Volume | ≤ 150 mg |
Purity | A260/A280 nm ≥1.8. |
Sample Source | Leaves, stems, buds, flowers, fruit, seeds, etc. |
Sample Storage | DNA stored at ≤ -20°C. |
Size Range | Capable of recovering genomic DNA up to and above 40 kb. Typical fragment sizes range from 25 kb – 35 kb. If present, parasitic and viral DNA will also be recovered. |
Type | Total DNA |
Yield | ≤ 25 µg total DNA |
Kits Component
Cat # | Name | Size |
---|---|---|
D3004-1-100 | Genomic Lysis Buffer | 100 ml |
D3004-2-50 | g-DNA Wash Buffer | 50 ml |
D3004-4-10 | DNA Elution Buffer | 10 ml |
D3004-5-250 | DNA Pre-Wash Buffer | 250 ml |
D6001-3-40 | BashingBead Buffer | 40 ml |
D6035-1-30 | Prep Solution | 30 ml |
C1057-50 | Zymo-Spin III-F Filters | 50 Pack |
C1058-50 | Zymo-Spin III-HRC Filters | 50 Pack |
S6003-50 | ZR BashingBead Lysis Tubes (2 mm) | 50 Tubes |
C1078-50 | Zymo-Spin IICR Columns | 50 Pack |
PRODUCT FAQ
Q1: Can you provide a list of the tested plant species?
We currently do not have a list of plants, aside from what we have shown in the protocols: – A.thaliana – Juniper – Milkweed Leaf – Milkweed Leaflet – Milkweed Pre-Flowering Bud – Corn Kernel – Sunflower Seed – Nicotiana sp.
Q2: My lysate seems viscous. What is causing this to happen? How can I fix this?
A viscous sample can indicate incomplete sample lysis. Try using less of your sample and optimize bead beating conditions (duration, speed, time) to ensure samples are thoroughly lysed. After bead beating, pellet the cell debris before moving on. Adding more Genomic Lysis buffer to the lysate can help dilute and deproteinate the sample, making the sample less viscous and more suitable for DNA recovery.
Q3: Are there any tips in optimzing bead beating conditions?
We have validated our kits with both high-speed homgenizers and low-speed disruptors. We highly recommend users to optimize their bead beating conditions for their own instruments. We recommend using a 2 ml-tube adapter to ensure that the bead beating is efficent (do not use adapters made of foam). For high-speed homogenizers, we recommend a total of 5 mins bead beating (1 min interval at 6.5 m/s with 5 mins rest, repeat 5 times). For low-speed cell disruptors, we recommend 30 mins at max speed.
Q4: What is the purpose of Zymo-Spin III-HRC step?
Environmental samples often contain inhibitors such as polyphenolics, humic/fulvic acids, tannins, melanins, etc. that often co-purify and affect downstream applications such as PCR. The Zymo-Spin III-HRC column removes these polyphenolic PCR inhibitors to recover DNA that is ready for sensitive downstream applications such as Next-Gen Sequencing, quantitative PCR, etc.
Q5: Is it necessary to add beta-mercaptoethanol? Can this step be substituted or omitted?
Addition of beta-mercaptoethanol is recommended to enhance sample lysis, but can be substituted with dithiothreitol (DTT, final concentration of 10 mM). However, if bead beating is optimized and lysis is efficient, the addition of BME is not necessary and can be omitted.
Q6: When can an RNase A treatment be implemented in the protocol?
The Quick-DNA kits recover RNA-free genomic DNA. The selective chemistry allows for binding of double stranded DNA to the column and for RNA to flow through. No RNase A treatment is required when processing samples within kit capacity.
reviews
Miranda Karson
Pacific University
Jan 31 2022
Great results
DNA extraction worked well for end user. Thank you.
Application Area: PCR
Asela Wijeratne
Arkansas State University
Jan 31 2022
Great product, easy to use and very consistent
We routinely use this kit for DNA extraction from soybean samples and it is easy to use. Also, we get good quality DNA most of the time.
Application Area: Genomic DNA extraction
Roger Koide
Brigham Young University
Jan 31 2022
Good but could be improved to decrease user error and to make the kits complete.
The product is generally good. It is not a complete kit in that the necessary microfuge tubes are not included. Also, the labeling of the columns is particularly awkward. Better to name them column 1, column 2, column 3 to minimize the possibility of screwing up.
Application Area: Identification of fungi and bacteria in plant tissues
Ethan Faryna
Kansas State University
Dec 16 2021
Good price, effective DNA isolation.
Good price, effective DNA isolation.
Application Area: Plant SNP analysis
Christina Eddy
North Greenville University
Nov 16 2021
I always use ZymoResearch products for DNA isolation.
I use this product with undergraduate biology students, just learning aseptic technique and micro pipetting. We achieve high-quality DNA close to 100 percent of the time. The components are clearly labeled, instructions are easy to follow and the PCR products are easily obtained from the purified DNA.
Application Area: Bacterial DNA isolation from plant material to analyze for endophytes