Quick-DNA Plant/Seed Miniprep Kit


Cat # Name Size
D6020 Quick-DNA Plant/Seed Miniprep Kit 50 Preps


  • Rapid and simple method for DNA isolation from tough-to-lyse plant and seed samples.
  • Ultra-high density BashingBeads are fracture resistant and chemically inert.
  • Zymo-Spin column technology coupled with filtration removes polyphenolic PCR inhibitors from the DNA product.

The Quick-DNA Plant/Seed Miniprep Kit is a plant DNA isolation kit designed for the simple, rapid isolation of inhibitor-free, PCR-quality DNA from a variety of plant sample sources including leaves, stems, buds, flowers, fruit, seeds, etc. The procedure is easy and can be completed in minutes: plant samples are rapidly and efficiently lysed by bead beating with our state of the art, ultra-high density BashingBeads. Polysaccharides, lipids, and polyphenols/tannins are removed from the DNA using our Zymo-Spin column technology. The eluted DNA is ideal for downstream molecular-based applications including PCR, arrays, etc.

Technical Specifications
Applicable ForAll sensitive downstream applications such as qPCR and Next-Generation Sequencing.
Elution Volume≥ 50 µl
EquipmentMicrocentrifuge, vortex, cell disruptor/pulverizer
Processing Volume≤ 150 mg
PurityA260/A280 nm ≥1.8.
Sample SourceLeaves, stems, buds, flowers, fruit, seeds, etc.
Sample StorageDNA stored at ≤ -20°C.
Size RangeCapable of recovering genomic DNA up to and above 40 kb. Typical fragment sizes range from 25 kb – 35 kb. If present, parasitic and viral DNA will also be recovered.
TypeTotal DNA
Yield≤ 25 µg total DNA

Kits Component

Cat # Name Size
D3004-1-100 Genomic Lysis Buffer 100 ml
D3004-2-50 g-DNA Wash Buffer 50 ml
D3004-4-10 DNA Elution Buffer 10 ml
D3004-5-250 DNA Pre-Wash Buffer 250 ml
D6001-3-40 BashingBead Buffer 40 ml
D6035-1-30 Prep Solution 30 ml
C1057-50 Zymo-Spin III-F Filters 50 Pack
C1058-50 Zymo-Spin III-HRC Filters 50 Pack
S6003-50 ZR BashingBead Lysis Tubes (2 mm) 50 Tubes
C1078-50 Zymo-Spin IICR Columns 50 Pack


Q1: Can you provide a list of the tested plant species?

We currently do not have a list of plants, aside from what we have shown in the protocols: – A.thaliana – Juniper – Milkweed Leaf – Milkweed Leaflet – Milkweed Pre-Flowering Bud – Corn Kernel – Sunflower Seed – Nicotiana sp.

Q2: My lysate seems viscous. What is causing this to happen? How can I fix this?

A viscous sample can indicate incomplete sample lysis. Try using less of your sample and optimize bead beating conditions (duration, speed, time) to ensure samples are thoroughly lysed. After bead beating, pellet the cell debris before moving on. Adding more Genomic Lysis buffer to the lysate can help dilute and deproteinate the sample, making the sample less viscous and more suitable for DNA recovery.

Q3: Are there any tips in optimzing bead beating conditions?

We have validated our kits with both high-speed homgenizers and low-speed disruptors. We highly recommend users to optimize their bead beating conditions for their own instruments. We recommend using a 2 ml-tube adapter to ensure that the bead beating is efficent (do not use adapters made of foam). For high-speed homogenizers, we recommend a total of 5 mins bead beating (1 min interval at 6.5 m/s with 5 mins rest, repeat 5 times). For low-speed cell disruptors, we recommend 30 mins at max speed.

Q4: What is the purpose of Zymo-Spin III-HRC step?

Environmental samples often contain inhibitors such as polyphenolics, humic/fulvic acids, tannins, melanins, etc. that often co-purify and affect downstream applications such as PCR. The Zymo-Spin III-HRC column removes these polyphenolic PCR inhibitors to recover DNA that is ready for sensitive downstream applications such as Next-Gen Sequencing, quantitative PCR, etc.

Q5: Is it necessary to add beta-mercaptoethanol? Can this step be substituted or omitted?

Addition of beta-mercaptoethanol is recommended to enhance sample lysis, but can be substituted with dithiothreitol (DTT, final concentration of 10 mM). However, if bead beating is optimized and lysis is efficient, the addition of BME is not necessary and can be omitted.

Q6: When can an RNase A treatment be implemented in the protocol?

The Quick-DNA kits recover RNA-free genomic DNA. The selective chemistry allows for binding of double stranded DNA to the column and for RNA to flow through. No RNase A treatment is required when processing samples within kit capacity.


5 review stars

Average Rating of 4.8/5.0

Based on 10 reviews

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Miranda Karson

Pacific University

Jan 31 2022

4 review stars

Great results

DNA extraction worked well for end user. Thank you.

Application Area: PCR

Asela Wijeratne

Arkansas State University

Jan 31 2022

5 review stars

Great product, easy to use and very consistent

We routinely use this kit for DNA extraction from soybean samples and it is easy to use. Also, we get good quality DNA most of the time.

Application Area: Genomic DNA extraction

Roger Koide

Brigham Young University

Jan 31 2022

4 review stars

Good but could be improved to decrease user error and to make the kits complete.

The product is generally good. It is not a complete kit in that the necessary microfuge tubes are not included. Also, the labeling of the columns is particularly awkward. Better to name them column 1, column 2, column 3 to minimize the possibility of screwing up.

Application Area: Identification of fungi and bacteria in plant tissues

Ethan Faryna

Kansas State University

Dec 16 2021

5 review stars

Good price, effective DNA isolation.

Good price, effective DNA isolation.

Application Area: Plant SNP analysis

Christina Eddy

North Greenville University

Nov 16 2021

5 review stars

I always use ZymoResearch products for DNA isolation.

I use this product with undergraduate biology students, just learning aseptic technique and micro pipetting. We achieve high-quality DNA close to 100 percent of the time. The components are clearly labeled, instructions are easy to follow and the PCR products are easily obtained from the purified DNA.

Application Area: Bacterial DNA isolation from plant material to analyze for endophytes