ZymoBIOMICS DNA Miniprep Kit


Cat # Name Size
D4300 ZymoBIOMICS DNA Miniprep Kit (Lysis Tubes) 50 Preps
D4300T ZymoBIOMICS DNA Miniprep Kit 5 Preps
D4304 ZymoBIOMICS DNA Miniprep Kit (No Lysis Matrix) 50 Preps


  • ✓ Validated Unbiased for Microbiome Measurements: Unbiased cellular lysis validated using the ZymoBIOMICS Microbial Community Standard.
  • ✓ Inhibitor-Free DNA from Any Sample: Isolate ultra-pure DNA ready for any downstream application.
  • ✓ Certified Low Bioburden: Boost your detection limit for low abundance microbes.

The ZymoBIOMICS DNA Kits are microbial DNA purification kits designed for purifying DNA from a variety of sample inputs that is immediately ready for microbiome or metagenome analyses. The ZymoBIOMICS lysis system eliminates bias associated with unequal lysis efficiencies of different organisms (e.g. Gram-negative/positive bacteria, fungus, protozoans, and algae) making it ideal for microbial community profiling. Uniform mechanical lysis of all microbes is achieved by bead beating with the innovative ultra-high density BashingBeads. This kit is equipped with our OneStep PCR Inhibitor removal technology, enabling PCR amplification from DNA derived from inhibitor-rich environmental samples. Purified DNA is ideal for all downstream applications including PCR, arrays, 16S rRNA gene sequencing, and shotgun sequencing. DNA Size is 15-20 kb.

Technical Specifications
Applicable ForAll sensitive downstream applications such as qPCR and Next-Generation Sequencing.
Elution Volume≥ 50 µl DNA Elution Buffer or DNase free water
EquipmentMicrocentrifuge, vortex/Disruptor Genie, high speed cell disruptor (recommended).
Processing VolumeFecal ≤200 mg, Soil ≤ 250 mg, Cells ≤ 100 mg (Approximately equal to 2×109 bacterial, 2×108 yeast, or 2×107 mammalian cells)
PurityHigh quality, inhibitor-free DNA. Typical A260/A280 ≥1.8
Sample SourceBacterial, fungal, protozoan, algae, viral, mitochondrial, and host DNA is efficiently isolated from feces, soil, fungal/bacterial cells, biofilms, and water.
Sample StorageEluted DNA should be stored at ≤ -20°C.
Size RangeTypically 15-20 kb post-bead beating. For optimal DNA integrity, collect samples in DNA/RNA Shield.
TypeTotal DNA
YieldUp to 25 µg total DNA can be eluted into ≥50 µl


Kits Component

Cat # Name Size
C1058-50 Zymo-Spin III-HRC Filters 50 Pack
S6012-50 ZR BashingBead Lysis Tubes (0.1 & 0.5 mm) 50 Tubes
C1078-50 Zymo-Spin IICR Columns 50 Pack
D4300-1-40 ZymoBIOMICS Lysis Solution 40 ml
D4300-2-100 ZymoBIOMICS DNA Binding Buffer 100 ml
D4300-3-50 ZymoBIOMICS DNA Wash Buffer 1 50 ml
D4300-7-30 ZymoBIOMICS HRC Prep Solution 30 ml
D4300-4-60 ZymoBIOMICS DNA Wash Buffer 2 60 ml
D4302-5-1 ZymoBIOMICS DNase/RNase Free Water 1 ml
D4302-5-10 ZymoBIOMICS DNase/RNase Free Water 10 ml
C1057-50 Zymo-Spin III-F Filters 50 Pack


5 review stars

Average Rating of 4.7/5.0

Based on 74 reviews

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Archana Kumari

Rowan University School of Osteopathic Medicine

Jul 28 2021

5 review stars

Easy to use.

Achieved high quality results, easy to use.

Application Area: DNA isolation from ChIP samples

Matthew Brock

UT Southwestern Medical Center

Jul 28 2021

5 review stars

This extraction kit works great and is very easy to use.

This kit is very easy to use and works great for most microbial samples. We use it mostly for bacterial extraction from stool samples. The gDNA collected afterward is great for 16s sequencing or shotgun metagenomics. This is our go to kit for most samples.

Application Area: Microbiome extraction

Isis Mejias

Global WaSH

Jul 28 2021

5 review stars

Fast and reliable results.

The kit is very easy to use and effective. This is my number 1 kit. Instructions to use are very clear and concise.

Application Area: DNA extraction

Neha Mittal

University of North Carolina at Charlotte

Jul 28 2021

4 review stars

Amazing quality results/kit/chemicals.

This kit has a great combination of chemicals that can provide good quality and quantity. Easy to use with straightforward protocol to be use. I use it almost everyday and very happy with its performance. Highly recommended.

Application Area: Extraction kit

Zhuoteng Yu

Precidiag Inc

Jul 28 2021

5 review stars

Easy to handle, good results.

This DNA/RNA Kit is not very consistent for the faecal microbiome. RNA output is too low to detect. But it is easy to handle.

Application Area: Analyzing microbiome DNA