• Flexible RT-qPCR kit compatible with both, SYBR Green and TaqMan based assays
  • Efficient cDNA synthesis and robust amplification in as little as 1.5 hours
  • Superior performance

ZymoScript One-Step RT-qPCR Kit is an optimized RT-qPCR kit that contains all the necessary components for fast reverse transcription and robust qPCR amplification in a single tube. The One-Step RT-qPCR kit is compatible with both SYBR Green and TaqMan probe-based assays for ultimate flexibility. The visual tracking dye included in the master mix allows accurate reaction set-up.

Technical Specifications
Equipment thermal cycler/qPCR instrument
Sample Source RNA
Size 100 reactions


The amount of total RNA required may vary depending on the expression level of the target transcripts. In general, we recommend using 0.1 pg – 5 µg of input RNA.

The dye used in the ZymoScript One-Step RT-qPCR Kit has been thoroughly tested in downstream analysis. At the provided concentration, it will not affect fluorescent signal readings during qPCR.

Lower signal can be expected with degraded and impure RNA samples. We recommend using Quick-RNA or Direct-zol Kits (see Related Products) for high quality RNA extraction.

No, only use gene specific primers when using ZymoScript One-Step RT-qPCR Kit.

Yes, the reaction can be assembled at room temperature. The enzymes are only activated at higher temperatures, ensuring maximum specificity.

We do not recommend storing the kit or kit components at room temperature for prolonged periods of time. The kit can be stored at 4°C for up to 1 week.

Samples can be treated with DNase I to eliminate DNA contamination. DNase I is included in the Quick-RNA or Direct-zol Kits recommended for RNA extraction. Alternatively, the DNase I set (see Related Products) can be purchased separately.

No, RNase inhibitors are already included in the enzyme mix to prevent unwanted RNA degradation during reverse transcription.

This depends on different factors, including RNA integrity and primer efficiencies. Generally, this product performs the best with amplicons with a length between 50 bp and 1.5 Kb.

Kit Components

Cat # Name Size
W1001-1 DNase/RNase-Free Water 1 ml

EZ-96 DNA Methylation-Lightning Kit

Cat # Name Size
R3014 ZymoScript One-Step RT-qPCR Kit 100 reactions