•   Efficient and quick cDNA synthesis (in less than 15 minutes)
  • Simple, ready-to-use-2X formulation
  • Visual tracking dye for accurate reaction set-up

ZymoScript RT PreMix is an optimized reverse transcription kit containing all the necessary components for quick and efficient cDNA synthesis. This reverse transcription mix includes random hexamer and oligo dT primers, dNTPs, RNase inhibitors, reverse transcriptase, and an optimized reaction buffer which includes a visual tracking dye for accurate reaction set-up. This is the kit of choice for analysis of RNA via 2-step RT-qPCR.

Technical Specifications
Equipment thermal cycler
Sample Source RNA
Size Range 100 reactions


The amount of total RNA required may vary depending on the expression level of the target transcripts. In general, we recommend using 0.1 pg – 5 µg of input RNA.

The dye used in ZymoScript RT PreMix has been thoroughly tested in downstream analyses. At the provided concentration, it will not affect fluorescent signal readings of any qPCR fluorescent channel.

Lower cDNA yields can be expected with degraded and/or impure RNA samples. We recommend using Quick-RNA or Direct-zol Kits (see Related Products) for high quality RNA extraction.

Yes, gene specific primers can be used to increase specific target yield. However, we recommend using ZymoScript One Step RT-qPCR (see Related Products) for enhanced specificity.

Yes, the enzymes are not activated at ambient temperatures.

We do not recommend storing the kit at room temperature for prolonged periods of time. The RT PreMix can be stored at 4°C for up to 1 week.

Samples can be treated with DNase I to eliminate DNA contamination. DNase I is included in the Quick-RNA and Direct-zol Kits recommended for RNA extraction (see Related Products). Alternatively, the DNase I set can be purchased separately.

No. The ZymoScript RT PreMix (2X) already contains a mixture of oligo dT and random hexamer primers which is not ideal for the synthesis of long cDNA molecules. For this specific application we recommend using the ZymoScript One-Step RT-qPCR Kit (R3014) with gene-specific primers.

Kit Components

Cat # Name Size
W1001-1 DNase/RNase-Free Water 1 ml

EZ-96 DNA Methylation-Lightning Kit

Cat # Name Size
R3012 ZymoScript RT PreMix Kit 100 reactions