Protein Extraction and Purification

BioVision's Protein Extraction and Purification Product Line contains antibiotics, cell lysis agents for bacterial cells, cell fraction kits, detergents, phosphatase and protease inhibitor cocktails, tag antibodies and protein quantitation kits. The fraction kits allow for separation of cytosolic, organellar, mitochondrial, nuclear, plasma membrane and cytoskeletal fractions. In addition, kits are included for isolation of genomic and/or mitochondrial DNA. Our detergent line includes high quality nonionic detergents (NP-40, Triton X-100, Tween-20, Tween-80, OG, OTG), a zwittergent detergents (CHAPS, ASB-14, Sulfobetaine 3-16) and an ionic detergent (SDS). The phosphatase and protease inhibitor cocktails are offered as ready to use formulations and encompass a wide variety and combinatorial forms which include both phosphatase and protease inhibitors. Our tag antibody line includes GST-, His-, HA-, S-, CBP-, DYDDDDK- and Myc-tag antibodies, while the anti-HA-tag antibodies are also offered with biotin or FITC conjugates. Finally, our protein quantification kits include both BCA-based and Bradford-type protein analysis.

Protein Extraction and Purification Products:

EZLabel™ Protein Cy5 Labeling Kit (BioVision Inc.)

Easy to use Kit to label upto 5 mg of Protein with Cy5 for multiple applications such as ELISA, western blot, Immunohistochemistry, Immunofluorescence and FACS analysis

EZLabel™ Protein Cy3 Labeling Kit (BioVision Inc.)

Easy to use Kit to label upto 5 mg of Protein with Cy3 for multiple applications such as ELISA, western blot, Immunohistochemistry, Immunofluorescence and FACS analysis

Maleimide Activated KLH-Peptide Conjugation Kit (BioVision Inc.)

Maleimide Activated KLH is a commonly used carrier protein for haptens, (small or low molecular weight molecules and peptides used to enable the immune response). Maleimide Activated KLH is the most popular carrier protein used for antibody production

Maleimide Activated BSA-Peptide Conjugation Kit (BioVision Inc.)

Bovine Serum Albumin (BSA) is a commonly used carrier protein in antibody production. The haptens, small molecules or peptides, are normally conjugated to BSA for enhancing their immune response.

Immobilized Catalase Beads (BioVision Inc.)

An ideal solution to limit the hydrogen peroxide interferance from sample analytes in downstream analyses.

Hi-Bind™ Albumin-IgG Depletion Beads (BioVision Inc.)

An ideal affinity purification matrix to deplete human albumin and IgG from plasma and serum samples for down stream processing

EZSolution™ Protein Stabilizer (BioVision Inc.)

A ready-to-use protein formulation to increase the stability of inactive proenzymes and active enzymes under chemical or thermal stress.

EZExtract™ Polyubiquitin Buffer Kit (BioVision Inc.)

Set of buffers and reagents required for the extraction of ubiquitinated or sumoylated proteins

EZDetect™ Polyubiquitin Probe (Biotin conjugated) (BioVision Inc.)

BioVision’s EZDetect™ Polyubiquitin Probe is ideal for detecting poly-ubiquitinated proteins in biological samples. Ubiquitin is a highly conserved 76-amino acid protein. It can be conjugated via its C-terminus to the amine groups of lysine residues on target proteins. This conjunction is referred to as monoubiquitylation.

EZCatch™ GST-Spin Purification Kit (BioVision Inc.)

Contains 10 columns and sufficient reagents for easy & efficient purification of a total of ~2.5 mg of recombinant GST-fusion proteins from 10 different samples.