Protein Extraction and Purification

BioVision's Protein Extraction and Purification Product Line contains antibiotics, cell lysis agents for bacterial cells, cell fraction kits, detergents, phosphatase and protease inhibitor cocktails, tag antibodies and protein quantitation kits. The fraction kits allow for separation of cytosolic, organellar, mitochondrial, nuclear, plasma membrane and cytoskeletal fractions. In addition, kits are included for isolation of genomic and/or mitochondrial DNA. Our detergent line includes high quality nonionic detergents (NP-40, Triton X-100, Tween-20, Tween-80, OG, OTG), a zwittergent detergents (CHAPS, ASB-14, Sulfobetaine 3-16) and an ionic detergent (SDS). The phosphatase and protease inhibitor cocktails are offered as ready to use formulations and encompass a wide variety and combinatorial forms which include both phosphatase and protease inhibitors. Our tag antibody line includes GST-, His-, HA-, S-, CBP-, DYDDDDK- and Myc-tag antibodies, while the anti-HA-tag antibodies are also offered with biotin or FITC conjugates. Finally, our protein quantification kits include both BCA-based and Bradford-type protein analysis.

Protein Extraction and Purification Products:

HA-Tag Antibody (Clone 12CA5) (BioVision Inc.)

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. The HA tag is derived from an epitope of the influenza hemagglutinin protein, which has been extensively used as a general epitope tag in expression vectors.

GST-Tag Antibody (BioVision Inc.)

Glutathione S-transferase (GST) is a widely used protein fusion partner, since it provides an easily detectable Tag and also a simple purification process with little effect on the biological function of the protein of interest.

GFP Antibody (BioVision Inc.)

Green fluorescent protein (GFP) is a spontaneously fluorescent protein isolated from pacific jellyfish, Aequorea victoria. It transduces the blue chemiluminescence into green fluorescent light.

C-Myc Antibody (BioVision Inc.)

Epitope tags are usually for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein’s biochemical properties.

Antibody/Protein Aggregation Assay Kit (BioVision Inc.)

Rapid & simple assay to measures total protein aggregation

EZCut™ HRV14 3C (Precission) Protease Agarose Beads (BioVision Inc.)

EZCut™ HRV14 3C (Precission) Protease Agarose is prepared by covalent coupling active HRV14 3C Protease to activated 4% cross-linked agarose beads.

Core Streptavidin (BioVision Inc.)

A non-glycosylated protein which is widely used to bridge biotynlated probes and enzymes.

Ammonium sulfate saturated solution (BioVision Inc.)

Convenient, ready to use sterile solution for Purification/Fractionation of proteins

7” Polypropylene Disposable Gravity Column (BioVision Inc.)

Disposable polyproplyene columns for general chromatography

EZLabel™ Protein FITC Labeling Kit (BioVision Inc.)

Easy to use Kit to label upto 5 mg of Protein with FITC for multiple applications such as ELISA, western blot, Immunohistochemistry, Immunofluorescence and FACS analysis