BioVision’s EZLabel™ Protein-Cy5 Labeling Kit provides an easy way to label proteins with Cy5 in a user-friendly spin column format. Cy5 is an ideal fluorescent dye for labeling of proteins with its intense signal in the red region of the spectrum. Each pair of spin columns provided in the kit can be used to purify up to 1 mg of the labeled target protein. The kit provides all of the reagents necessary to perform five labeling reactions using up to 1 mg of protein per reaction. The Cy5-labeled protein has excitation and emission wavelengths at 650 nm and 670 nm respectively, and can be directly used for downstream applications including ELISA, western blot, Immunohistochemistry, Immunofluorescence, and FACS, etc.
|Cat # +Size||K837-5|
|Size||For 5 x 1 mg of protein|
|Detection Method||Absorbance at 280 nm and 550 nm|
|Applications||Labeled proteins can be used for ELISA, western blot, Immunohistochemistry, Immunofluorescence, and FACS analysis|
|Features & Benefits||All necessary reagents included. Fast and efficient labeling of proteins with Cy3|
|Kit Components||• EZLabel™ Cy3 • EZLabel™ Spin Column • EZLabel™ Elution Buffer|
|Shipping Conditions||Gel Pack|
|USAGE||For Research Use Only! Not For Use in Humans.|
What is the principle of Cy5 labelling in K839?
The principle of this kit is based on amine modification. Labeling methodology involves reacting of primary amines with sulfo-NHS Cy5 ester thereby forming an amide bond. As the amine groups are distributed throughout the protein, the resulting conjugate may have the label distributed randomly across the protein.
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