Isolation and Sample Preparation Kits

Isolation and Sample Preparation Kits Products:

Mitochondria/Cytosol Fractionation Kit (BioVision)

The Mitochondria/Cytosol Fractionation Kit provides unique formulations of reagents for effective isolation of a highly enriched mitochondrial fraction from cytosolic fraction of mammalian cells including both apoptotic and nonapoptotic cells.

Microsome Isolation Kit (BioVision)

Microsomes are spherical vesicle-like structures formed from membrane fragments following homogenization and fractionation of eukaryotic cells. The microsomal subcellular fraction is prepared by differential centrifugation and consists primarily of membranes derived from the endoplasmic reticulum (ER) and Golgi apparatus.

Membrane Protein Extraction Kit (BioVision)

The Membrane Protein Extraction Kit provides optimized buffers and reagents for effective extraction of membrane proteins from mammalian tissues and cells. Unlike other available procedures that can only extract the total cellular membrane proteins

Mammalian Mitochondria Isolation Kit For Tissue & Cultured Cells (BioVision)

Mitochondria are the powerhouse of the cells because they generate most of the supply of energy in the form of adenosine tri-phosphate (ATP). Mitochondria are double membrane organelles: an outer membrane and a folded inner membrane called cristae. Isolated mitochondria are the sample of choice to study mitochondrial respiration, assembly of the respiratory complexes, apoptosis, mtDNA and mtRNA, and mitochondrial protein profiling. The kit offers two options for the isolation of intact mitochondria. The first option is utilizing a reagent-based method allowing parallel processing of multiple samples. The second option uses traditional dounce homogenization, which provides better mitochondrial yield.

Mammalian Cell & Tissue Extraction Kit (BioVision)

The Mammalian Cell & Tissue Extraction Kit provides optimized cell extraction buffer, protease inhibitor cocktail, and DTT for convenient extraction of mammalian proteins from cultured cells and tissue samples, under nondenaturing conditions. Cell lysate prepared using the kit can be used in a variety of applications, such as enzyme activity assays (e.g., caspase activity assays), Western blot analysis, and others. The entire procedure takes less than 20 minutes.

Lysosome Purification Kit (BioVision)

Lysosomes are membrane-bound cell organelles found in most animal cells (except red blood cells). Lysosomes are spherical vesicles containing hydrolytic enzymes capable of breaking down different kinds of biomolecules, including proteins, nucleic acids, carbohydrates, lipids, and cellular debris.

HDAC3 Immunoprecipitation (IP) & Activity Assay Kit (BioVision)

Histone deacetylases (HDACs) play a central role in controlling cell cycle regulation, cell differentiation, and tissue development. These proteins have crucial roles in development and physiology, especially in the heart and nervous system.

HDAC2 Immunoprecipitation (IP) & Activity Assay Kit (BioVision)

Histone deacetylases (HDACs) play a central role in controlling cell cycle regulation, cell differentiation, and tissue development. HDAC1 is ubiquitously expressed and plays a role in cellular senescence, cardiomyocyte differentiation and hypertrophy,

HDAC1 Immunoprecipitation (IP) & Activity Assay Kit (BioVision)

Histone deacetylases (HDACs) play a central role in controlling cell cycle regulation, cell differentiation, and tissue development. HDAC1 is ubiquitously expressed and plays a role in cellular senescence, cardiomyocyte differentiation and hypertrophy,

Genomic DNA Isolation Kit (BioVision)

The Genomic DNA Isolation Kit provides a simple and convenient procedure for rapid isolation of genomic DNA from mammalian cells and tissue samples with high yield and purity. The novel method requires less than 90 minutes to prepare highly pure genomic DNA.