Lysosomes are membrane-bound cell organelles found in most animal cells (except red blood cells). Lysosomes are spherical vesicles containing hydrolytic enzymes capable of breaking down different kinds of biomolecules, including proteins, nucleic acids, carbohydrates, lipids, and cellular debris. They are known to contain over 50 different enzymes, which are active under acidic conditions. Thus, lysosomes act as the waste disposal system of the cells by digesting unwanted materials in the cytoplasm. BioVision’s Lysosome Isolation Kit provides a procedure for isolating enriched or purified lysosomal fraction from animal tissues and cultured cells by differential centrifugation followed by density gradient centrifugation
|Cat # +Size||K235-50|
|Applications||Isolation of intact functional lysosomes from tissues and cultured cells Lysosome studies like enzyme activity or uptake studies Protein profiling of Lysosomes by SDS-PAGE and Western blot|
|Features & Benefits||• Simple & convenient protocol
• The kit provides unique formulations of ready-to-use buffers and reagents to isolate lysosomes with minimal sample preparation
|Kit Components||• Lysosome Isolation Buffer
• Lysosome Enrichment Buffer
• Lysosome Gradient
• Protease Inhibitor Cocktail
|Shipping Conditions||Gel Pack|
|USAGE||For Research Use Only! Not For Use in Humans.|
Can we use flash frozen tissue for isolating lysosomes?
No. Isolation of lysosomes is recommended from fresh samples and not frozen samples.
I only have 10 ml ultracentrifuge tubes and not 2 ml tubes as suggested in the datasheet. Can I use a 10 ml ultracentrifuge tube instead of a 2 ml volume?
Yes, 10 ml tube can be used. But for preparing the density gradient for a 10 ml tube, the volumes given for 2 ml gradient must be multiplied by factor 5. Also, we recommend increasing the starting cell number from 2 X10^7 to 3x10^7.
What is the exact volume of sample required for this assay?
There is no specific volume we can recommend for the amount any sample to be used since it is completely sample concentration and quality based. You have to do a pilot expt with multiple sample volumes to determine the optimal volume which gives a reading within the linear range of the standard curve. Please refer to the citations for this product to see what other clients have used with similar sample types.
Do you have trial sizes of this kit?
Unfortunately, we do not have trial sizes of this kit available. However, if you are based in the US or Canada, we will give you a 10% off list price introductory discount on its purchase price. If you are based out of this area please contact yopur regional BioVision distributor.
Can we purchase individual components of this kit?
Yes, you can purchase any of the kit's components without the whole kit. Please refer to the component Cat #s mentioned on the datasheet for ordering.
Can we use an alternate buffer for sample preparation (cell lysis, sample dilutions etc)?
Our assay buffers are optimized for the reactions they are designed for. They not only contain some detergents for efficient lysis of your cells/tissue, but also contain some proprietary components required for the further reactions. Therefore, we highly recommend using the buffers provided in the kit for the best results.
High Quality Guaranteed Product
Our products such as Elisa, Antibodies, Proteins, Peptides and sequencing kits are covered by Biolinkk quality warranty and will work as described in datasheet, a free replacement or money back is guaranteed if does not perform according to datasheet.