Description

The Genomic DNA Isolation Kit provides a simple and convenient procedure for rapid isolation of genomic DNA from mammalian cells and tissue samples with high yield and purity. The novel method requires less than 90 minutes to prepare highly pure genomic DNA. The extracted genomic DNA is free from protein and RNA, and suitable for a variety of applications such as PCR, DNA hybridization, enzyme manipulation, cloning, Southern blot, and array-based experiments.

Datasheet

FAQ

How can I measure the DNA amount or purity after the last step?
Generally 20-50 ug DNA can be obtained following the kit procedure. You can either take 1/3 - 1/2 of the solution and add water to 100 ul to measure it using 100 ul micro-cuvet, or alternatively, dilute the whole amount of DNA into 100 ul or 1 ml cuvet for measuring and then recover it by isopropanol precipitation.

Does the kit remove mitochondrial DNA?
The kit does not remove the mitochondrial DNA. If you want to remove mitochondrial DNA, you can lyse the cells in 0.5% NP-40 in Tris Buffer (pH8.0), then centrifuge and isolate nuclei pellet (mitochondrial DNA will be in supernatant). Then follow the kit procedure.

I wanted to clarify whether or not the following product can isloate nuclear DNA from mitochondrial DNA in birds.
We have not tested your specific application. However, the genomic DNA isolation kit can be used in any cells or tissue samples. This kit is different from column based DNA isolation kit. In this kit, proteins or RNAs is degraded and DNA is precipitated. The kit can recover all DNAs from your samples.

Can this kit be used with samples like bacteria, plants, drosophila, yeast etc?
We have optimized the kit with mammalian samples. However, theoretically these kits should work with samples from multiple species/sources. Since the optimal conditions depend on the sample type, the protocol has to be be adapted to fit the samples for efficient results. Please refer to this kit's citations to see what kind of samples have been used with this kit other than mammalian samples.

Can we use frozen samples with this assay?
Fresh samples are always preferred over frozen samples. However, frozen samples can also be used, provided, they were frozen right after isolation, were not freeze thawed multiple time (for which we recommend aliquoting the samples before freezing) and have been frozen for relatively short periods.

What is the exact volume of sample required for this assay?
There is no specific volume we can recommend for the amount any sample to be used since it is completely sample concentration and quality based. You have to do a pilot expt with multiple sample volumes to determine the optimal volume which gives a reading within the linear range of the standard curve. Please refer to the citations for this product to see what other clients have used with similar sample types.

Do you have trial sizes of this kit?
Unfortunately, we do not have trial sizes of this kit available. However, if you are based in the US or Canada, we will give you a 10% off list price introductory discount on its purchase price. If you are based out of this area please contact your regional BioVision distributor.

What is the shelf life of this kit?
This kit is good for 12 months from the date of shipment in the unopened form when stored at the appropriate temperature and appropriate conditions. After opening and reconstitution, some of the components in this kit are good for 2 months at -20°C. Please refer to the datasheet for storage information and shelf life of each of the components.

Can we purchase individual components of this kit?
Yes, you can purchase any of the kit's components without the whole kit. Please refer to the component Cat #s mentioned on the datasheet for ordering.

Can we use an alternate buffer for sample preparation (cell lysis, sample dilutions etc)?
Our assay buffers are optimized for the reactions they are designed for. They not only contain some detergents for efficient lysis of your cells/tissue, but also contain some proprietary components required for the further reactions. Therefore, we highly recommend using the buffers provided in the kit for the best results.

CITATIONS
García-Ruiz et al., In vitro treatment of HepG2 cells with saturated fatty acids reproduces mitochondrial dysfunction found in nonalcoholic steatohepatitis. Dis. Model. Mech.,Feb 2015; 8: 183 - 191.

Kim Y et al. (2007) Cancer Res. 67: 3583 - 3593.

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