Tag peptides are short amino acid sequences that are added to a protein of interest to facilitate detection, purification, or localization within a cell. These peptides serve as molecular markers or “tags” that can be easily recognized by specific antibodies or enzymes for various experimental purposes.
Tag peptides are commonly used in molecular biology and biochemistry research to track the expression and behavior of proteins within cells. They are often fused to the N- or C-terminus of a target protein, allowing researchers to easily monitor its movement, interaction with other proteins, or localization within specific cellular compartments.
Some commonly used tag peptides include the FLAG tag, Myc tag, and HA tag, each with their own unique features and advantages for different experimental applications. The versatility and specificity of tag peptides make them invaluable tools for studying protein function and cellular signaling pathways.
Overall, tag peptides are a subcategory of signaling pathways that play a crucial role in the study of protein localization, function, and interactions within cells. Their use has greatly advanced our understanding of molecular biology and cellular processes.
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