The RNA Clean & Concentrator kits are RNA clean up kits that provide a simple and reliable method for the rapid preparation of high-quality RT-PCR-ready, DNA-free (R1013, R1014) RNA. This simple procedure is based on the use of a unique single-buffer system and Zymo-Spin column technology that allows for selective recovery of total RNA (> 17 nt), large RNAs (> 200 nt), and/or small RNAs (17-200 nt). The procedure is easy: Add binding buffer and ethanol to your sample, then bind, wash and elute ultra pure RNA. The RNA can be eluted from the Zymo-Spin IC Column in as little as = 6 µl of RNase-free water. The highly-concentrated, purified RNA is suitable for all subsequent analyses and molecular manipulations.

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Equipment	Microcentrifuge
Format	Spin-Column
Purity	RNA is ready for Next-Gen sequencing, RT-PCR, microarray, hybridization, etc. A260/A280, A260/A230: > 1.8.
Sample Source	DNase I treated RNA, in vitro transcription products, the aqueous phase following TRIzol/chloroform or similar extraction
Size Range	Total RNA ≥ 17 nt
Yield	250 µg RNA (binding capacity), ≥ 100 µl (elution volume)

Q1: Can the RCC kit be used to purify DNA (cDNA)?

Q2: Should carrier RNA be added to the sample prior to purification?

Q3: Will this kit remove fluorescent dyes, radiolabeled dNTP’s and/or Biotin?

Q4: Are samples containing high concentration of detergents, salts, formamide or sucrose compatible with the RCC buffer system?

Q5: Can other DNase I enzymes or sets (e.g. Qiagen DNase I) be used?

Q6: Is DNase I treatment necessary?

Q7: Which protocol is recommended? In-tube or in-column DNase treatment?

“Great for purifying small amounts of RNA. I use a bioanalyzer after and I can see the difference from before, cleaner bands and the concentration increases with a smaller amount of elutant.”

– Catherine A. (USAMRIID)

“Kit was easy and fast, and cleaned up my RNA samples very well. It did exactly what I needed it to do.”

– Caroline S. (Cornell University)

“The product is very easy to use, very quick protocol for an excellent RNA clean up. It also purifies small RNA which is not the case of all RNA clean up columns.”

– Agnes N. (Biofidal)

“Zymo RNA Clean & Concentrator is the best product for CRISPR applications. We have tried several other products including precipitation methods, Zymo product gave us the best results and yield.”

– Gaurav V. (OMRF)

“Great result. Everybody should try this product. I’ve never seen such purity of the RNA before. This product works excellent and easy to use and affordable.”

– Para S. (University of Louisville)

“I used this as a clean-up step after guanidinium thiocyanate-phenol-chloroform extraction from homogenized tissues. I found the process very simple, and adding the kit on did seem to significantly improve the quality of the RNA pool relative to the original phenol-chloroform extraction.”

– Emily G. (University of Wisconsin-Madison)