RCC1 Monoclonal Antibody
- Size: 100ul
- Application: WB
- Reactivity:
- Predicted Reactivity: Human, Mouse
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Datasheet
Tech Support
IMAGES
Lane 1: HL-60 cell lysates; Lane 2: Hela cell lysates; Lane 3: U-251MG cell lysates; Lane 4: MCF-7 cell lysates; Lane 5: Mouse Heart tissue lysates probed with RCC1 Monoclonal Antibody, Unconjugated (bsm-51500M) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
Lane 1: HL-60 cell lysates probed with RCC1 Monoclonal Antibody, Unconjugated (bsm-51500M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
PRODUCT DETAILS
SPECIFICATIONS |
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|---|---|
|
PRODUCT NAME |
RCC1 Monoclonal Antibody |
|
CONJUGATION |
Unconjugated |
|
HOST |
Mouse |
| SOURCE |
Recombinant human RCC1. |
| IMMUNOGEN RANGE |
|
| CLONALITY |
Monoclonal |
| ISOTYPE |
IgG1 |
| CONCENTRATION |
1ug/ul |
| PURIFICATION |
|
| STORAGE BUFFER |
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| STORAGE CONDITION |
Shipped at 4_. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
TARGET |
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|---|---|
| GENE ID |
|
| SUBCELLULAR LOCATION |
Cytoplasm, Nucleus |
| SYNONYMS |
Cell cycle regulatory protein; CHC 1; CHC1; Chromosome condensation 1; Chromosome condensation protein 1; Guanine nucleotide releasing protein; HERC2; Ran GEF; RanGEF; RCC 1; RCC1 I; Regulator of chromosome condensation 1; Regulator of chromosome condensation; SHEP1; RCC1_HUMAN. |
| BACKGROUND |
Guanine-nucleotide releasing factor that promotes the exchange of Ran-bound GDP by GTP. Involved in the regulation of onset of chromosome condensation in the S phase. Binds both to the nucleosomes and double-stranded DNA. RCC1-Ran complex (together with other proteins) acts as a component of a signal transmission pathway that detects unreplicated DNA. Plays a key role in nucleo-cytoplasmic transport, mitosis and nuclear-envelope assembly. |
| APPLICATION DILUTION |
WB(1:300-1000) |