RNAzol® BD Column Kit
Full Protocol (PDF) | SDS (PDF)
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Catalog# RC 292
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The RNAzol® BD Column Kit combines RNAzol® BD, with a versatile and universal column in one procedure. This Kit provides unsurpassed yields of either total RNA, large RNA (> 150 – 200 bases) or small RNA (< 150 – 200 bases) in separate fractions. RNAzol® BD has been used to determine that RNA averages 14 μg/ml of human whole blood and ranges from 6 – 22 μg/ml. These RNA levels are significantly higher than previously reported with other methods that average only 2 – 5 μg of total RNA per ml of human blood
• This Kit allows for isolation of low RNA quantities without pellets and in about 60 minutes.
• The large RNA fraction includes long non-coding RNA, mRNA and rRNA.
• The small RNA fraction includes tRNA, small rRNA and microRNA down to 10 bases.
• The Kit allows for simultaneous isolation of RNA and DNA from the same sample.
• The pure and undegraded RNA is ready for RT-PCR without DNase treatment.
• RNAzol® BD Column Kit isolates pure and undegraded RNA from whole blood, plasma or serum of human or animal origin.
Catalog # RC 292: 50 ml RNAzol® BD, 50 Columns with Collection Tubes, 50 Wash Tubes, 50 Elution Tubes, 10 ml RNase-free Water
Catalog # RC 292T: Trial size available-10 isolations
1. US patents 7,794,932 and 8,367,817. International patents.
Full Protocol (PDF)
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