Description

Exosomes are vesicles secreted by cells. They are present in a variety of body fluids such as serum, ascites, spinal cord fluid, urine, and saliva. Cultured cells also secrete significant number of exosomes. The size distribution of exosome is ranging from 30-120 nm. The biological function of exosomes is believed to serve as intercellular messengers. Filtration and ultracentrifugation are classical ways of isolating and enriching exosomes. This is a tedious process and requires special equipment. Another common way for enrichment of exosome is through precipitation. Currently major commercial kits are PEG-based. EI-027 is designed to precipitate total exosomes from biofluids using a high efficacy, non-PEG based reagent for exosome precipitation. This kit is suitable for routine biofluid samples using the same reagent and similar protocol.

Kit Includes

References

1. Bai, H., Pan, Y., Qi, L., Liu, L., Zhao, X., Dong, H., ... & Wang, X. (2018). Development a hydrazide-functionalized thermosensitive polymer based homogeneous system for highly efficient N-glycoprotein/glycopeptide enrichment from human plasma exosome. Talanta.
2. Jiao, F., Xia, C., Zhao, Y., Ying, W., Xie, Y., Guan, X., ... & Qian, X. (2018). A novel strategy for facile serum exosome isolation based on specific interactions between phospholipid bilayers and TiO2. Chemical Science.
3. Tan, Y., Nie, W., Chen, C., He, X., Xu, Y., Ma, X., ... & Wang, W. (2019). Mesenchymal stem cells alleviate hypoxia-induced oxidative stress and enhance the pro-survival pathways in porcine islets. Experimental Biology and Medicine, 1535370219844472.

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