Minute™ Detergent-Free Nuclear Matrix Protein Extraction Kit (For Mammalian Cultured Cells/Tissues) (20 Preps)


Minute™ Detergent-Free Nuclear Matrix Protein Extraction Kit (For Mammalian Cultured Cells/Tissues) (20 Preps)


Manual & Protocol (PDF)   |   Material Safety Data Sheets (MSDS)(PDF)

View All Related Products




Catalog# NM-033

Size:


Price Not Available

Contact Us




Description




Nucleoplasm, also referred to as karyoplasm/nucleus sap, consists of chromosomes, nucleolus and nuclear matrix. Nucleoplasm plays a central role in genetic information flow and regulation of gene expression. Nuclear protein isolation has traditionally been accomplished by extraction protocol using detergent-containing buffers, which is effective in extracting total nuclear proteins but unable to separate membrane-bound proteins and water-soluble matrix proteins. The detergent-free nuclear matrix protein extraction kit is designed to separate cellular protein into three fractions: cytosolic, nuclear matrix and water-insoluble fractions (mainly envelope and nucleic acid-associated proteins) using a patented spin-column-based technology. The buffers used are detergent and EDTA-free. The whole protocol can be completed in less than 1 hour. The proteins extracted are in their native state and can be used in many downstream applications such as gel retardation, transcription factor analysis, apoptosis and protein trafficking studies.












Kit Includes




Items Quantity
Buffer A 30 ml
Buffer B 5 ml
Protein Extraction Filter Cartridges 20 units
Collection Tubes 20 units
Plastic Rods 2 units




References




-






Post Reviews


Leave a Reply

Your email address will not be published. Required fields are marked *






We're here to help


Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.


Contact Tech Support







High Quality Guaranteed Product


Our products such as Elisa, Antibodies, Proteins, Peptides and sequencing kits are covered by Biolinkk quality warranty and will work as described in datasheet, a free replacement or money back is guaranteed if does not perform according to datasheet.


Learn More