Minute™ Cytosolic Proteasome Enrichment Kit (20 preps)

Minute™ Cytosolic Proteasome Enrichment Kit (20 preps)

Manual & Protocol (PDF)   |   Material Safety Data Sheets (MSDS)(PDF)

View All Related Products

Catalog# PT-040


For orders and inquiries

Contact Us


The proteasome is a non-lysosome protein degradation system that requires metabolic energy and polymerization of ubiquitin. The 26S proteasome is made up of two subcomplexes (a 20S proteasome and one or two19S regulatory particles). Rapid and gentle isolation of proteasome from cells/tissues is essential for the studies of proteasome structure and function. The proteasomes have been found mainly localized in cytosol but also detected in nucleus. Traditionally, ultracentrifugation and affinity isolation are the most common methods for isolation of proteasomes. These methods, though relatively effective, are usually time consuming with low yield. Many affinity-based methods require harsh elution condition that may affect the activity of isolated proteasomes and limit certain downstream applications. To overcome these shortcomings, we have developed a spin-column based technology using a proprietary precipitation buffer for enrichment of proteasome from cytosol by first removing nuclei and majority of organelles followed by preferential precipitation of cytosolic proteasomes. The nuclear proteasomes are excluded with this kit (Nuclear Proteasome Enrichment Kit is available under Cat # PN-041). The protocol is simple and rapid with high yield. The gentle protocol maintains the association of proteasomes with ubiquitin and other proteins. This kit is useful for the studies of proteasome structure and function. The enriched proteasomes can also be used as a starting material for affinity purification.

Kit Includes

Items Quantity
Buffer A 10 ml
Buffer B 10 ml
Protein Extraction Filter Cartridges 20 Units
Collection Tubes with caps 20 Units
Plastic Rods 2 Units



Post Reviews

Leave a Reply

Your email address will not be published. Required fields are marked *

We're here to help

Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

Contact Tech Support

High Quality Guaranteed Product

Our products such as Elisa, Antibodies, Proteins, Peptides and sequencing kits are covered by Biolinkk quality warranty and will work as described in datasheet, a free replacement or money back is guaranteed if does not perform according to datasheet.

Learn More