Minute™ Cytosolic and Nuclear Extraction Kit for Frozen/Fresh Tissues (20 Preps)


Minute™ Cytosolic and Nuclear Extraction Kit for Frozen/Fresh Tissues (20 Preps)


Manual & Protocol (PDF)   |   Material Safety Data Sheets (MSDS)(PDF)

View All Related Products




Catalog# NT-032

Size:


Price Not Available

Contact Us




Description




Preparation of cytosolic and nuclear fractions from cultured cells and tissues is common. Cell fractionation from cultured cells is relatively easy and straightforward. However, a clear separation of cytosolic and nuclear fractions from tissues, especially frozen tissues, is challenging. The cross-contamination from frozen tissues is always an issue because of the altered tissue cellular structure caused by the freeze and thaw cycle, improper homogenization, and the use of an inadequate extraction buffer. The majority of current commercial kits are designed to treat cultured cells and tissues similarly, not paying sufficient attention to the structural difference between the two. As a result, cross-contamination of cytosolic and nuclear fractions from fresh/frozen tissues remains a major problem (see references 1 and 2 below). This novel Cytosolic and Nuclear Extraction Kit is specially designed to address the issue by employing proprietary buffers and unique protocol to minimize the cross-contamination for fresh and frozen tissue samples.












Kit Includes




Items Quantity
Buffer A 25 ml
Buffer B 10 ml
Buffer D 1.5 ml
Buffer N 1.5 ml
Pestle for 1.5 ml microfuge tube 2 Units
Protein Extraction Powder 2 g




References




-






Post Reviews


Leave a Reply

Your email address will not be published. Required fields are marked *






We're here to help


Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.


Contact Tech Support







High Quality Guaranteed Product


Our products such as Elisa, Antibodies, Proteins, Peptides and sequencing kits are covered by Biolinkk quality warranty and will work as described in datasheet, a free replacement or money back is guaranteed if does not perform according to datasheet.


Learn More