Biotin Anti-Mouse IgG2c rabbit monoclonal antibody [RM223]
Datasheet
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Catalog#: 31-1103-02
Applications: WB (nonreduced only), IP, ICC, IHC, FC, ELISA
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Product Details
Product Specifications |
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Product Name |
Biotin Anti-Mouse IgG2c rabbit monoclonal antibody [RM223] |
| Product Description: |
Biotinylated Rabbit monoclonal to Mouse IgG2C |
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Catalog No. |
31-1103-02 |
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Clone Name |
RM223 |
| Product Datasheet |
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| Specificity |
This antibody reacts to the Fc region of mouse IgG2c.No cross reactivity with mouse IgG1, IgG2a, IgG2b,IgG3, IgM, IgA, IgE, human IgG, or rat IgG. |
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Immunogen |
Mouse IgG2c |
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Application |
WB (nonreduced only), IP, ICC, IHC, FC, ELISA |
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Species Reactivity |
Mouse |
| Conjugate |
Biotin |
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Intended Use |
Research Use Only |
Properties |
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Host |
Rabbit |
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Storage Instruction |
Store at -20.0°C |
| Stability |
Stable for 1 Year at -20.0°C from date of receipt |
Storage Buffer |
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
Concentration |
1 mg/mL |
Volume/Size |
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| Purity |
Protein A affinity purified from an animal origin-free culture supernatant |
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Clone Type |
Recombinant Monoclonal |
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Isotype |
Rabbit IgG |
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Uniport |
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Gene ID |
404711 |
| Usage |
ELISA: 0.01 ug/mL – 0.2 ug/mLImmunocytochemistry (ICC): 0.5 ug/mL-2 ug/mLImmunohistochemistry (IHC): 0.5 ug/mL-2 ug/mLWestern Blot (WB): 0.5 ug/mL-2 ug/mL |
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Keywords |
- |
*For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Product Images
ELISA of IgG from BalB/C and C57BL6 shows RM223 reacts to C57BL6 IgG containing IgG2c, and does not react to BalB/C IgG containing IgG2a. 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM223 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
A titer ELISA of mouse IgG2c. The plate was coated with different amounts of mouse IgG2c. A serial dilution of RM223 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
Western blot of nonreduced(-) and reduced(+) mouse IgG2c, using 0.5ug/mL of RevMAb clone RM223. This antibody reacts to nonreduced IgG2c