Anti-Phospho-Akt (Ser473) rabbit monoclonal antibody [RM251]
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Product Details
Product Specifications |
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Product Name |
Anti-Phospho-Akt (Ser473) rabbit monoclonal antibody [RM251] |
Product Description: |
Rabbit monoclonal to phospho-Akt (Ser473), AKT1 (phospho S473) |
Catalog No. |
31-1131-00 |
Clone Name |
RM251 |
Product Datasheet |
Anti-Phospho-Akt (Ser473) rabbit monoclonal antibody [RM251] |
Specificity |
This antibody reacts to Akt only when phosphorylated at Ser473. There is no cross-reactivity with Akt without phosphorylation at Ser473. This antibody may also react to bovine, mouse or rat Phospho-Akt (Ser473), as predicted by immunogen homology |
Immunogen |
A phospho-peptide corresponding to human Phospho-Akt (Ser473) |
Application |
WB, IHC |
Species Reactivity |
Human, (Bovine, Mouse, Rat) |
Conjugate |
None |
Intended Use |
Research Use Only |
Properties |
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Host |
Rabbit |
Storage Instruction |
Store at -20.0°C |
Stability |
Stable for 1 Year at -20.0°C from date of receipt |
Storage Buffer |
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
Concentration |
N/A |
Volume/Size |
Anti-Phospho-Akt (Ser473) rabbit monoclonal antibody [RM251] |
Purity |
Protein A affinity purified from an animal origin-free culture supernatant |
Clone Type |
Recombinant Monoclonal |
Isotype |
Rabbit IgG |
Uniport |
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Gene ID |
207 , 208 , 10000 |
Usage |
Immunohistochemistry (IHC): 1:200-1:500 dilution;Western Blot (WB): 1:1000-1:2000 dilution. |
Keywords |
- |
*For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Product Images
Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue sections using Anti-Phospho-Akt (Ser473) RM251 at a 1:400 dilution.
Western Blot of lysates from 293 cells either, grown in medium with serum, serum starved, or insulin treated. Using Anti-Phospho-Akt (Ser473) RM251 at a 1:1000 dilution or anti-Akt1 RM252 at a 1:1000 dilution.