Anti-Gamma H2AX (phospho-Ser139) Rabbit Monoclonal Antibody [RM224]
Datasheet
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Catalog#: 31-1104-00
Applications: WB, ELISA, Multiplex, ICC
Price Not Available
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Product Details
Product Specifications |
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Product Name |
Anti-Gamma H2AX (phospho-Ser139) Rabbit Monoclonal Antibody [RM224] |
| Product Description: |
Rabbit monoclonal to Gamma H2AX (phospho-Ser139) |
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Catalog No. |
31-1104-00 |
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Clone Name |
RM224 |
| Product Datasheet |
Anti-Gamma H2AX (phospho-Ser139) Rabbit Monoclonal Antibody [RM224] |
| Specificity |
This antibody reacts to Histone H2A.X only when phosphorylated at serine 139. No cross reactivity with other phosphorylated histones. |
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Immunogen |
A phospho-peptide corresponding to Phospho-Histone H2AX (Ser139) |
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Application |
WB, ELISA, Multiplex, ICC |
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Species Reactivity |
Human Vertebrates |
| Conjugate |
None |
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Intended Use |
Research Use Only |
Properties |
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Host |
Rabbit |
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Storage Instruction |
Store at -20.0°C |
| Stability |
Stable for 1 Year at -20.0°C from date of receipt |
Storage Buffer |
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
Concentration |
1 mg/mL |
Volume/Size |
Anti-Gamma H2AX (phospho-Ser139) Rabbit Monoclonal Antibody [RM224] |
| Purity |
Protein A affinity purified from an animal origin-free culture supernatant |
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Clone Type |
Recombinant Monoclonal |
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Isotype |
Rabbit IgG |
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Uniport |
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Gene ID |
3014 |
| Usage |
Western Blot: 0.5 ug/mL – 2 ug/mL;ELISA: 0.2 ug/mL - 1 ug/mL;Multiplex: 0.1 ug/mL – 1 ug/mL;ICC: 0.5 ug/mL - 2 ug/mL. |
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Keywords |
- |
*For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Product Images
Western Blot of acid extracts of HeLa cells treated or non-treated with UV. Using RM224 at 0.5 ug/mL, showed a band of Histone H2A.X phosphorylated at serine 139 in HeLa cells.
Immunocytochemistry of HeLa cells using gamma H2AX Rabbit mAb RM224 (red). Actin filaments have been labeled with fluorescein phalloidin (green).