Anti-Dimethyl Histone H3 (Lys23) rabbit monoclonal antibody [RM171]
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Catalog#: 31-1077-00
Applications: WB, ELISA, Multiplex
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Product Details
Product Specifications |
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Product Name |
Anti-Dimethyl Histone H3 (Lys23) rabbit monoclonal antibody [RM171] |
Product Description: |
Rabbit monoclonal to Dimethylated Histone H3 Lysine 23, H3K23me2 |
Catalog No. |
31-1077-00 |
Clone Name |
RM171 |
Product Datasheet |
Anti-Dimethyl Histone H3 (Lys23) rabbit monoclonal antibody [RM171] |
Specificity |
This antibody reacts to Histone H3 dimethylated at Lysine 23 (K23me2). No cross reactivity with monomethylated Lysine 23 (K23me1) or trimethylated Lysine 23 (K23me3), or other methylation in histone H3. |
Immunogen |
A dimethyl-peptide corresponding to Dimethyl-Histone H3 (Lys23). |
Application |
WB, ELISA, Multiplex |
Species Reactivity |
Human Vertebrates |
Conjugate |
None |
Intended Use |
Research Use Only |
Properties |
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Host |
Rabbit |
Storage Instruction |
Store at -20.0°C |
Stability |
Stable for 1 Year at -20.0°C from date of receipt |
Storage Buffer |
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
Concentration |
1 mg/mL |
Volume/Size |
Anti-Dimethyl Histone H3 (Lys23) rabbit monoclonal antibody [RM171] |
Purity |
Protein A affinity purified from an animal origin-free culture supernatant |
Clone Type |
Recombinant Monoclonal |
Isotype |
Rabbit IgG |
Uniport |
|
Gene ID |
8350 |
Usage |
Western Blot: 0.5 ug/mL – 2 ug/mL;ELISA: 0.1 ug/mL - 0.5 ug/mL;Multiplex: 0.1 ug/mL – 0.5 ug/mL. |
Keywords |
- |
*For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Product Images
A Peptide dotblot shows RM171 only reacts to Histone. H3 dimethyl-Lysine 23 (K23me2). No cross reactivity. with nonmodified Lysine 23 (K23Ctrl), monomethylated. Lysine 23 (K23me1), or dimethylated Lysine 4 (K4me2).
RM171 specifically reacts to Histone H3 dimethylated at Lysine 23 (K23me2). No cross reactivity with other methylated lysines in Histone H3.
Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using RM171 at 0.5 ug/mL, showed a band of histone H3 dimethylated at Lysine 23 (K23me2) in HeLa cells.