Anti-Dimethyl Histone H3 (Lys18) rabbit monoclonal antibody [RM168]
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Catalog#: 31-1075-00
Applications: WB, ELISA, Multiplex
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Product Details
Product Specifications |
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Product Name |
Anti-Dimethyl Histone H3 (Lys18) rabbit monoclonal antibody [RM168] |
Product Description: |
Rabbit monoclonal to Dimethylated Histone H3 Lysine 18, H3K18me2 |
Catalog No. |
31-1075-00 |
Clone Name |
RM168 |
Product Datasheet |
Anti-Dimethyl Histone H3 (Lys18) rabbit monoclonal antibody [RM168] |
Specificity |
This antibody reacts to Histone H3 dimethylated at Lysine 18 (K18me2). No cross reactivity with monomethylated Lysine 18 (K18me1) or trimethylated Lysine 18 (K18me3), or other methylation in histone H3. |
Immunogen |
A dimethyl-peptide corresponding to Dimethyl-Histone H3 (Lys18). |
Application |
WB, ELISA, Multiplex |
Species Reactivity |
Human Vertebrates |
Conjugate |
None |
Intended Use |
Research Use Only |
Properties |
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Host |
Rabbit |
Storage Instruction |
Store at -20.0°C |
Stability |
Stable for 1 Year at -20.0°C from date of receipt |
Storage Buffer |
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
Concentration |
1 mg/mL |
Volume/Size |
Anti-Dimethyl Histone H3 (Lys18) rabbit monoclonal antibody [RM168] |
Purity |
Protein A affinity purified from an animal origin-free culture supernatant |
Clone Type |
Recombinant Monoclonal |
Isotype |
Rabbit IgG |
Uniport |
|
Gene ID |
8350 |
Usage |
Western Blot: 0.2 ug/mL – 1 ug/mL;ELISA: 0.1 ug/mL - 0.5 ug/mL;Multiplex: 0.1 ug/mL – 0.5 ug/mL. |
Keywords |
- |
*For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Product Images
A Peptide dotblot shows RM168 only reacts to Histone. H3 dimethyl-Lysine 18 (K18me2). No cross reactivity. with nonmodified Lysine 18 (K18Ctrl), monomethylated. Lysine 18 (K18me1), dimethylated Lysine 4 (K4me2),. or dimethylated Lysine 36 (K36me2).
RM168 specifically reacts to Histone H3 dimethylated at Lysine 18 (K18me2). No cross reactivity with other methylated lysines in Histone H3.
Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using RM168 at 0.5 ug/mL, showed a band of histone H3 dimethylated at Lysine 18 (K18me2) in HeLa cells.