Product Details
Product Specifications |
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Product Name |
Anti-His-Tag Chimeric Human Monoclonal Antibody, [RMH01] |
| Product Description: |
Chimeric Human monoclonal to His-Tag. (6xHis-Tag or 10xHis-Tag) at either the N-Terminus (amino) or C-Terminus (carboxy) |
|
Catalog No. |
54-1161-00 |
|
Clone Name |
RMH01 |
| Product Datasheet |
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| Specificity |
This antibody is a Human-Rabbit chimeric antibody, made by fusion of the antigen binding region (variable domains of the heavy and light chains, VH and VL) of rabbit anti-HisTag monoclonal antibody (RM146), with the constant domain of Human IgG1. The antibody is recognized only by an anti-Human IgG secondary antibody. |
|
Immunogen |
Mixture of a peptide with 6xHis-Tag at the N-terminus and another peptide with 6xHis-Tag at the C-terminus. |
|
Application |
WB, ELISA |
|
Species Reactivity |
N/A |
| Conjugate |
None |
|
Intended Use |
Research Use Only |
Properties |
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|---|---|
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Host |
Rabbit |
|
Storage Instruction |
Store at -20.0°C |
| Stability |
Stable for 1 Year at -20.0°C from date of receipt |
Storage Buffer |
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
Concentration |
1 mg/mL |
Volume/Size |
|
| Purity |
Protein A affinity purified from an animal origin-free culture supernatant |
|
Clone Type |
Recombinant Monoclonal |
|
Isotype |
Chimeric |
|
Uniport |
|
|
Gene ID |
N/A |
| Usage |
ELISA: Capturer: 0. 1 ug/mL – 2 ug/mL; Detector: 0.01 ug/mL – 0.5 ug/mL;Western Blot: 0.1 ug/mL – 1 ug/mL. |
|
Keywords |
- |
*For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Product Images
Sandwich ELISA performed on different amounts of whole lysate of 293T cells transfected with a DNA construct encoding HisTag Histone H3 (K36M) protein, using 50uL/well of different concentrations of chimeric human anti-HisTag antibody Clone RMH01 as the capture antibody. Anti-H3K36M rabbit monoclonal antibody (RM193) was used as the detection antibody, followed by an alkaline phosphatase conjugated Rabbit IgG as the Secondary antibody.
A titer ELISA performed on purified HisTag-PCSK9 recombinant protein. A serial dilution of chimeric human anti-HisTag antibody Clone RMH01 was used as the primary antibody. An alkaline phosphatase conjugated anti-Human IgG as the secondary antibody.
Western blot of 293T cells transfected (+) or untransfected (-) with a DNA construct encoding His-Tag Histone H3 (G34W) protein, using chimeric human anti-HisTag antibody Clone RMH01 at 0.2ug/mL, followed by a HRP conjugated anti-Human IgG secondary antibody.