- Highest Yield: Purify up to 100 µg of plasmid DNA in as little as 25 µl directly from a spin-column.
- Transfection-Grade: 50,000 times fewer endotoxins than industry leading minipreps.
- BAC/YAC/PAC Ready: Purify DNA up to ~200 kb.
|Applicable For||Transfection, transformation, sequencing, restriction endonuclease digestion, in vitro transcription, in vivo studies, genome editing, and other sensitive applications.|
|Binding Capacity||100 µg|
|Culture Input||≤ 5 mL|
|Elution Volume||25 µl|
|Endotoxin Levels||≤ 1 EU/µg|
|Equipment||Microcentrifuge and/or vacuum manifold|
|Processing Time||15 min|
|Purity||Typical Abs 260/280 ≥1.8 and Abs 260/230≥ 2.0|
|Recommended Sample Volume||≤ 5.0 ml|
|Size Range||Up to 25 kb|
|Yield||Up to 100 µg per preparation, depending on the plasmid copy number, culture growth conditions, and strain of E. coli utilized.|
Q1: What is the composition of the ZymoPURE™ Elution buffer?
Q2: I ran out of ZymoPURE™ Wash 2. Can I substitute it with a homemade solution or Wash Buffer from another kit?
Q3: What type of vacuum pump do you recommend?
Q4: Are the ZymoPURE kits compatible with any commercially available vacuum manifold?
Q5: Can an in-house vacuum line be used with the EZ Vac Vacuum Manifold?
Q6: Can the ZymoPURE Kits be used with other bacteria?
Q7: Is there a protocol for low-copy number plasmid DNA?
Q8: Can I process more than the recommended volume of bacterial culture or use enriched growth media?
Q9: Can the ZymoPURE kits be used to isolate large plasmid constructs (BAC/PAC)?
ZymoPURE Plasmid Miniprep Kit