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- ZymoPURE 96 Plasmid Miniprep Kit
highlights
- High-Throughput: Perform 96 Plasmid Minipreps Simultaneously using a Vacuum Manifold or Centrifuge.
- Highest Yield: Up to 100 μg of Highly Concentrated Plasmid DNA.
- Ultra-Pure: Eluted Plasmid DNA is Transfection-Grade and Free of Cross-Contamination.
The ZymoPURE™ 96 Plasmid Miniprep Kit features a high-throughput method for the purification of up to 100 µg of ultra-pure transfection-grade plasmid DNA using a vacuum manifold or centrifuge. The unique vacuum plate design provides zero buffer retention and low elution volumes. In addition, the provided wash plate also significantly reduces plasmid DNA cross-contamination between wells relative to other popular high-throughput plasmid prep kits.
ZymoPURE™ technology uses a modified alkaline lysis method and our patented binding chemistry, which enables the highest DNA binding capacity and rapid loading of the lysate and wash buffer, resulting in the purification of highly concentrated (up to 1 µg/µl) plasmid DNA directly from a 96-well vacuum plate. In addition, the wash regimen has been optimized to ensure the plasmid DNA is free of endotoxins, salt, protein, and RNA. The result is plasmid DNA suitable for transfection, transformation, lentivirus production, adenovirus production, AAV production, CRISPR, genome editing, sequencing, restriction endonuclease digestion, in vitro transcription/translation, PCR and other sensitive downstream applications.
As an added convenience, the ZymoPURE™ 96 Plasmid Miniprep Kit contains colored buffers that permit error-free visualization and identification of complete bacterial cell lysis and neutralization. ZymoPURE™ Filter Plates are also included for rapid clearing of the lysate and the unique Zymo-Spin P-96 Plate desig qn allows the binding step to be performed using a vacuum or centrifuge.
| Applicable For | Transfection, transformation, lentivirus production, adenovirus production, AAV production, CRISPR, genome editing, in vivo studies, sequencing, restriction endonuclease digestion, in vitro transcription/translation, PCR, and other sensitive applications. |
|---|---|
| Binding Capacity | 100 µg |
| Culture Input | ≤ 5 mL |
| Elution Volume | 125 µL |
| Endotoxin Levels | ≤ 1 EU/µg |
| Equipment | 96-well plate vacuum manifold (recommended) or swinging bucket centrifuge with microplate adapters. The vacuum pump should be a single or double-staged unit capable of producing up to 300 mm Hg at the vacuum manifold. |
| Format | 96-Well Plate |
| Processing Time | ≤ 60 min |
| Purity | Typical Abs260/280 ≥ 1.8 and Abs260/230 ≥ 2.0 |
| Recommended Sample Volume | ≤ 5.0 mL |
| Size Range | Up to ~200 kb |
| Yield | Up to 100 µg per preparation, depending on the plasmid copy number, culture growth conditions, and strain of E. coli utilized. |
FAQs
10 mM Tris-HCL, 0.1 mM EDTA, pH 8.5
No, the ZymoPURE™ kits are only compatible with ZymoPURE™ Wash 2 and we cannot disclose a substitution due to the sensitive nature of the recipe. Additional ZymoPURE™ Wash 2 can be purchased separately.
Yes, however, the pressure needs to be around 300 mm Hg. Users should take caution, as the pressure of an in-house vacuum line can fluctuate drastically or be significantly reduced, depending on the demand in the building.
Yes, the standard protocol should work with other gram-negative bacteria. However, the user will need to validate the protocol for their particular species. For gram-positive bacteria, please refer to the Gram-Positive Bacteria Protocol in the Appendix of the kit protocol. Appendix of the kit protocol.
Yes, the standard ZymoPURE protocol has been successfully tested with constructs up to 200 kb. To increase elution of large plasmid DNA, we recommend pre-warming the ZymoPURE Elution Buffer (50 ºC) and increasing the incubation time on column up to 10 minutes prior to centrifugation.
This product is compatible with any commercially available 96-well plate vacuum manifold that can process stacked plates. We recommend our EZ-Vac 96 Vacuum Manifold (S7003).
The vacuum pump should be a single or double-staged unit capable of producing at least 300 mm Hg pressure at the vacuum manifold.
Kit Components
| Cat # | Name | Size |
|---|---|---|
| D4200-1-100 | ZymoPURE P1 (Red) | 100 ml |
| D4200-2-100 | ZymoPURE P2 (Blue) | 100 ml |
| D4200-3-100 | ZymoPURE P3 (Yellow) | 100 ml |
| D4200-4-110 | ZymoPURE Binding Buffer | 110 ml |
| D4200-5-55 | ZymoPURE Wash 1 | 55 ml |
| D4200-6-23 | ZymoPURE Wash 2 (Concentrate) | 23 ml |
| D4200-7-30 | ZymoPURE Elution Buffer | 30 ml |
| C2022 | ZymoPURE Filter Plate | 2 x 96-well plate |
| C2023 | Zymo-Spin P-96 Plate | 2 x 96-Well Plate |
| C2024 | Wash Plate | 2 x 96-Well Plate |
| C2015 | 96-Well 2.0 mL Deep Well Plate | 2 plates |
| C2011-2 | Air Permeable Sealing Cover | 2 Pack |
| C2007-6 | 96-Well Plate Cover Foil | 6 Foils |
ZymoPURE 96 Plasmid Miniprep Kit
| Cat # | Name | Size |
|---|---|---|
| D4214 | ZymoPURE 96 Plasmid Miniprep Kit | 2 x 96 preps |
| D4215 | ZymoPURE 96 Plasmid Miniprep Kit | 4 x 96 preps |
*ZymoPURE 96 20% off Promotion Terms & Conditions: This promotion applies to direct US orders only and is one time use. 20% off ZymoPURE 96 SKUs: D4214, D4215. This promotion cannot be used with existing promotions or discounts. Other restrictions may apply. Promotional code is valid until 12/31/23.