SolisFAST® Probe qPCR Mix with UNG (ROX)
- Fast, highly sensitive and reproducible probe-based qPCR solution
Ultra fast probe-based qPCR Master Mix that includes novel in-silico designed SolisFAST® DNA Polymerase for robust and accurate target quantification. Suitable for UNG treatment to prevent cross-contamination.
✓ delivers qPCR results 2x faster (in as little as 30 minutes)
✓ reliable quantification in up to 5-plex assays
✓ consistent results with low- and high-copy targets
✓ robust performance
✓ contains dUTP and UNG to prevent cross-contamination
Catalog# 28-22-0000S
Size:0.2ml (50 rxn) 50 rxn Free sample
Size | Price |
0.2ml (50 rxn) | Get Quote |
OVERVIEW
Fast and sensitive ready-to-use 5X-concentrated solution for probe-based qPCR detection of DNA targets using TaqMan® and other hydrolysis probe types. The mix contains a novel SolisFAST® DNA Polymerase with fast hot-start and 2-4 times faster extension rates (15-30 sec/kb) compared to the wild-type Taq DNA polymerase (60 sec/kb) as well as HOT FIREPol® and FIREPol® DNA polymerases, and is optimised for sensitive detection of up to 5 targets in one reaction. It has three versions to match different instruments and assay requirements. ROX-mix is compatible with qPCR instruments that require low and high ROX level for signal normalization, Purple-mix is compatible with instruments that use Mustang Purple™ for signal normalisation. dUTP and UNG containing mixes are available upon request to prevent carry-over contamination. |
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DESCRIPTION |
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SolisFAST® Probe qPCR Mix is a 5x-concentrated, ready-to-use solution for fast, highly sensitive and reproducible probe-based qPCR assays using dual-labeled hydrolysis probes (e.g. TaqMan® probes), and is suitable for detection and quantitation of up to five targets simultaneously. |
KITS COMPONENT |
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• SolisFAST® DNA Polymerase: In silico- designed analog of Taq DNA polymerase with enhanced stability at room temperature, fast hot-start, and faster extension rates compared to the wild-type Taq DNA polymerase. |
STORAGE & SHIPPING |
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Storage: Routine storage at -20ºC until expiry date
The mix can be stored at +4ºC for up to 6 months
Stability at room temperature (15 − 25ºC) for 1 month
Freeze-thaw stability: 30 cycles |
REAGENT PROVIDED |
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CITATION
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