HOT FIREPol® Probe Universal qPCR Mix
- High performing probe-based qPCR Mix for AT-rich, regular and GC-rich templates
High performing probe-based qPCR Mix for AT-rich, regular and GC-rich templates.
✓ suitable for singleplex and duplex assays
✓ high specificity and sensitivity
✓ one qPCR mix for all cyclers (except capillary)
✓ superior results with templates with up to 75% GC content
✓ contains dUTP to prevent cross-contamination when used with UNG
✓ Benchtop stability for 48 hours for preassambled reactions
Catalog# 08-17-0000S
Size:0.2ml (50 rxn) 50 rxn Free sample
Size | Price |
0.2ml (50 rxn) | Get Quote |
OVERVIEW
A universal ready-to-use 5X reaction mix for probe-based qPCR detection of DNA targets using TaqMan® and other hydrolysis probe types. The mix is optimised for sensitive detection of up to 2 targets in one reaction with excellent amplification of GC-rich regions. The mix contains dUTPs to prevent carry-over contamination when used with UNG treatment, and an internal reference based on ROX dye and is compatible with most qPCR instruments, including those that require low and high ROX level for signal normalization. The mix is not compatible with Probes detected in ROX/JUN/Texas Red channel. |
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DESCRIPTION |
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HOT FIREPol® Probe Universal qPCR Mix is optimized for real-time quantitative PCR assays and contains all the components necessary to perform singleplex or duplex qPCR, with the exception of template, primers, and probes. Proprietary reaction buffer that enables efficient amplification of regular and GC-rich targets. |
KITS COMPONENT |
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• HOT FIREPol® DNA Polymerase: chemically modified FIREPol® DNA Polymerase enabling hot-start |
STORAGE & SHIPPING |
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Storage: Routine storage: -20ºC
Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of the product |
REAGENT PROVIDED |
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• HOT FIREPol® Probe Universal qPCR Mix 1x0.2 ml | 50 rxn |
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CITATION
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