A universal ready-to-use 5X reaction mix for probe-based qPCR detection of DNA targets using TaqMan® and other hydrolysis probe types. The mix is optimised for sensitive detection of up to 2 targets in one reaction with excellent amplification of GC-rich regions. The mix contains dUTPs to prevent carry-over contamination when used with UNG treatment, and an internal reference based on ROX dye and is compatible with most qPCR instruments, including those that require low and high ROX level for signal normalization. The mix is not compatible with Probes detected in ROX/JUN/Texas Red channel.

✓ suitable for assays with 1-2 targets
✓ high specificity and sensitivity
✓ superior results with templates with up to 75% GC content
✓ contains dUTP to prevent cross-contamination when used with UNG
✓ one qPCR mix for all cyclers (except capillary)
✓ reaction set-up and shipment without ice

HOT FIREPol® Probe Universal qPCR Mix is optimized for real-time quantitative PCR assays and contains all the components necessary to perform singleplex or duplex qPCR, with the exception of template, primers, and probes. Proprietary reaction buffer that enables efficient amplification of regular and GC-rich targets.
HOT FIREPol® Probe Universal qPCR Mix is optimized for DNA/LNA hydrolysis probes based on the 5' flap endonuclease activity.

The Mix contains internal passive reference dye that is compatible with most qPCR cyclers including high ROX or low ROX reference signal requiring platforms.

The Mix contains dUTPs to prevent cross-contamination with UNG treatment.

• HOT FIREPol® DNA Polymerase: chemically modified FIREPol® DNA Polymerase enabling hot-start
• 5x Probe Universal qPCR buffer with 15 mM MgCl2: 1x PCR solution – 3 mM MgCl2
• dNTPs including dUTP:  Mix allows UNG treatment to prevent carryover contamination from previous runs.
• IMPORTANT: UNG is not included in the HOT FIREPol® Probe Universal qPCR Mix
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• Reference dye based on ROX: For multiplex application - if ROX dye is used as one of the fluorophores, the internal reference might interfere with the signal.
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• In separate vial
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• 100% DMSO is included in the kit in a separate vial. DMSO is recommended as a PCR additive for templates with high GC content. In some cases, DMSO is also required to relax secondary structures.

Storage: Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of the product
Shipping: At room temperature

• HOT FIREPol® Probe Universal qPCR Mix 1x8 ml | 2000 rxn
• DMSO 100% 1x4 ml

Some applications of this product may require a license which is not provided by the purchase of this product.

**For research use only.