A cost-effective ready-to-use 5X-concentrated solution for probe-based qPCR detection of DNA targets using TaqMan® and other hydrolysis probe types. The mix is optimised for sensitive detection of up to 2 targets in one reaction. It has three versions to match different instruments. ROX-mix is compatible with qPCR instruments that require low or high ROX level for signal normalization. Capillary-mix is optimised for capillary based systems.

✓ cost-effective solution for less demanding assays
✓ suitable for assays with 1-2 targets
✓ high specificity and sensitivity
✓ compatible with most cyclers
✓ different product versions: no ROX, ROX, capillary
✓ reaction set-up and shipment without ice

HOT FIREPol® Probe qPCR Mix Plus (Capillary) is optimized for real-time quantitative PCR assays and contains all the components necessary to perform qPCR, with the exception of template, primers, and probe.
The qPCR Mix contains optimized components and HOT FIREPol® DNA Polymerase supplied in a proprietary reaction buffer that enables detection of low copy number targets. HOT FIREPol® Probe qPCR Mix Plus (Capillary) is optimized for DNA hydrolysis probes based on the 5´ flap endonuclease activity.

HOT FIREPol® DNA Polymerase is activated by a 12 min incubation step at 95°C. This prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.

• HOT FIREPol® DNA Polymerase: chemically modified FIREPol® DNA Polymerase enabeling hot-start
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• 5x Probe qPCR buffer with 15 mM MgCl2: 1x PCR solution – 3 mM MgCl2
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• dNTPs: dATP, dCTP, dGTP and dTTP
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• Bovine serum albumine (BSA) 

Storage: Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of the product
Shipping: At room temperature

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Some applications of this product may require a license which is not provided by the purchase of this product.

**For research use only.