A ready-to-use 5X-concentrated solution for probe-based qPCR detection of DNA targets using TaqMan® and other hydrolysis probe types. The mix is optimised for sensitive detection of up to 4 targets in one reaction with enhanced amplification of GC-rich regions. It contains dUTPs to prevent carry-over contamination when used with UNG treatment, and has three versions to match different instruments and assay requirements. ROX-mix is compatible with most qPCR instruments, including those that require low and high ROX level for signal normalization, Purple-mix is compatible with instruments that use Mustang Purple™ for signal normalisation.

✓ analyze 1-4 targets in 1 reaction
✓ high specificity and sensitivity
✓ robust amplification of GC-rich targets
✓ contains dUTP to prevent carry-over contamination when used in combination with UNG
✓ compatible with most cyclers (see table on page 17)
✓ different product versions: no ROX, ROX, Purple
✓ reaction set-up and shipment without ice

HOT FIREPol® Multiplex qPCR Mix is optimized for amplifying multiple targets in a single reaction in real-time quantitative PCR assays. The qPCR Mix comprises all the components necessary (except primers, probes and template) to perform qPCR: HOT FIREPol® DNA Polymerase, optimized buffer components, ultrapure dNTPs and MgCl2.


HOT FIREPol® Multiplex qPCR Mix is optimized for DNA hydrolysis probes based on the 5' flap endonuclease activity.


HOT FIREPol® DNA Polymerase is activated by a 10 min incubation step at 95°C. This prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.

• HOT FIREPol® DNA Polymerase: chemically modified FIREPol® DNA Polymerase enabeling hot-start
• 5x Multiplex qPCR buffer with 15 mM MgCl2: 1x PCR solution – 3mM MgCl2
• dNTPs including dUTP: the mix allows UNG treatment to prevent carryover contamination from previous runs.
• IMPORTANT: UNG is not included in the 5x HOT FIREPol® Multiplex qPCR Mix

Storage: Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of the product
Shipping: At room temperature

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Some applications of this product may require a license which is not provided by the purchase of this product.

**For research use only.