FIREScript® RT cDNA synthesis MIX without primers
- Convenient cDNA synthesis mix with fast and robust FIREScript® Reverse Transcriptase
All-inclusive and the most convenient product format for first-strand cDNA synthesis. A combined enzyme mix (FIREScript® and RiboGrip™) and a reaction premix in separate tubes allowing you to choose the most appropriate priming option.
✓ high specificity and yield
✓ wide reaction temperature from 37°C to 60°C
✓ fast 15 min reaction time
✓ a convenient 3-vial kit with all reaction components
✓ RNase inhibitor and water included
✓ reaction set-up and shipment without ice
OVERVIEW
FIREScript® is an improved version of Murine Moloney Leukemia Virus (M-MLV) reverse transcriptase (RT) with exceptional stability at room temperature, improved thermostability, substantially faster synthesis rates and higher sensitivity compared to the wild- ype M-MLV RT. FIREScript® is used for first strand cDNA synthesis from total RNA or purified mRNA and is compatible with wide range of reaction temperatures. |
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DESCRIPTION |
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FIREScript® Reverse Transcriptase (RT) is a genetically engineered MMLV (Moloney Murine Leukemia Virus) based Reverse Transcriptase. |
KITS COMPONENT |
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• FIREScript® Enzyme Mix (FIREScript® RT and RiboGrip RNase inhibitor) |
STORAGE & SHIPPING |
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Storage: Routine storage: -20ºC
Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of the product |
REAGENT PROVIDED |
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• FIREScript® enzyme mix 1x100 rxn | 150 µl |
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CITATION
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